Inhibition of p38 mitogen-activated protein kinase enhances c-Jun N-terminal kinase activity: implication in inducible nitric oxide synthase expression

BMC Pharmacology
Aleksi LahtiEeva Moilanen

Abstract

Nitric oxide (NO) is an inflammatory mediator, which acts as a cytotoxic agent and modulates immune responses and inflammation. p38 mitogen-activated protein kinase (MAPK) signal transduction pathway is activated by chemical and physical stress and regulates immune responses. Previous studies have shown that p38 MAPK pathway regulates NO production induced by inflammatory stimuli. The aim of the present study was to investigate the mechanisms involved in the regulation of inducible NO synthesis by p38 MAPK pathway. p38 MAPK inhibitors SB203580 and SB220025 stimulated lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression and NO production in J774.2 murine macrophages. Increased iNOS mRNA expression was associated with reduced degradation of iNOS mRNA. Treatment with SB220025 increased also LPS-induced c-Jun N-terminal kinase (JNK) activity. Interestingly, JNK inhibitor SP600125 reversed the effect of SB220025 on LPS-induced iNOS mRNA expression and NO production. The results suggest that inhibition of p38 MAPK by SB220025 results in increased JNK activity, which leads to stabilisation of iNOS mRNA, to enhanced iNOS expression and to increased NO production.

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Citations

Aug 20, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Li-Jin ChewVittorio Gallo
Mar 17, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Calivarathan LatchoumycandaneUrs A Boelsterli
May 8, 2008·Apoptosis : an International Journal on Programmed Cell Death·Melissa BragaIndrani Sinha-Hikim
Sep 14, 2007·Scandinavian Journal of Rheumatology·K VuolteenahoE Moilanen
Dec 30, 2014·Apoptosis : an International Journal on Programmed Cell Death·Yun-Ji LimChang-Hwa Song
Mar 27, 2015·American Journal of Physiology. Renal Physiology·Inge CarlsenRikke Nørregaard
May 23, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Sheryl Erica FernandesDeepak Kumar Saini
Oct 20, 2009·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Wioletta Ratajczak-WronaLeszek Piotrowski

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Methods Mentioned

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transfection
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