Inhibition of Paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis.

Microbiology
Gioconda San-BiasF San-Blas

Abstract

In Paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans, no significant differences were observed in the phospholipid species of both morphological phases. The species observed were phosphatidylcholine (PC, 30-40%), phosphatidylethanolamine (PE, 27-28%), phosphatidylserine (16-19%), phosphatidylinositol (13-17%) and sphingomyelin (3-5%). The main fatty acids found in the yeast (Y) phase were palmitate (56%), linoleate (18%) and oleate (15%), while linoleate predominated (61%) in the mycelial (M) phase, followed by palmitate (27%) and oleate (7%). In the Y phase the main free sterol was ergosta-5,22-dien-3 beta-ol (82%) plus some lanosterol (12%) and ergosterol (6%), while in the M phase, the latter predominated (88%), followed by low levels of ergosta-5,22-dien-3 beta-ol (12%). Ajoene [(E,Z)-4,5,9-trithiadodeca-1,6,11-triene 9-oxide], a platelet aggregation inhibitor derived from garlic, induced alterations in phospholipid and fatty acid proportions such that PC was reduced to about 18% in both phases and PE increased to 38% (Y phase) or 44% (M phase), suggesting inhibition of PC synthesis. Ajoene also reduced saturated fatty acids (16:0 and 18:0) from 67 to 35% in the Y phase, with a corresponding increase i...Continue Reading

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Citations

Dec 14, 1999·Microbes and Infection·S Ankri, D Mirelman
May 1, 2009·Annals of Clinical Microbiology and Antimicrobials·Lorena S DerengowskiIldinete Silva-Pereira
Feb 24, 2001·Drug Metabolism and Drug Interactions·L M Knowles, J A Milner
Apr 3, 2008·Expert Review of Anti-infective Therapy·Luiz R TravassosArnaldo L Colombo
Jun 4, 2011·Revista iberoamericana de micología·Joel Torres, Hilda Romero
Dec 21, 2010·Revista iberoamericana de micología·Gioconda San-Blas, Eva Burger
Jul 29, 2009·Revista iberoamericana de micología·Sarelie CarreroRafael Apitz-Castro
Dec 5, 2015·Frontiers in Microbiology·Haroldo C de OliveiraMaria J S Mendes-Giannini
Jan 5, 2010·Vaccine·Alice M RibeiroMaria Sueli S Felipe
Aug 20, 2019·PLoS Pathogens·Natália Ketelut-CarneiroJoão Santana Silva
Aug 28, 2019·Antimicrobial Agents and Chemotherapy·Ana Carolina Alves de Paula E SilvaMaria J S Mendes-Giannini
Apr 27, 2018·Frontiers in Microbiology·Beatriz GonzálezMaría-Jesús Torija
Oct 26, 2000·Journal of the American Academy of Dermatology·E LedezmaR Apitz-Castro

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