PMID: 6405735Feb 1, 1983Paper

Inhibition of pepsin by analogues of pepsinogen-(1-12)-peptide with substitutions in the 4-7 sequence region

The Biochemical Journal
B M DunnC Pham

Abstract

Derivatives of the 1-12 sequence of pig pepsinogen were prepared by solid-phase peptide synthesis. The three derivatives contain substitutions in the 4-7 region of the 1-12 sequence. Glycine was used to replace the hydrophobic residues -Val-Pro-Leu-Val- in pairs. After cleavage and purification, the synthetic peptides were compared with a synthetic peptide of the native sequence, prepared at the same time, with respect to their ability to inhibit the pepsin-catalysed clotting of milk. Inhibitory potency, determined from plots of percentage inhibition versus concentration of synthetic peptide, is inversely correlated with the substitution of glycine residues for the hydrophobic residues. Therefore the equilibrium inhibition of pepsin by these peptides is dominated by the hydrophobic nature of the 4-7 sequence region.

Citations

Jan 1, 1985·Scandinavian Journal of Gastroenterology. Supplement·J Calam
Feb 1, 1995·Protein Science : a Publication of the Protein Society·X LinJ Tang
Apr 18, 1994·FEBS Letters·G KoelschM Fusek
Jul 31, 1984·Biochimica Et Biophysica Acta·K M AraiK Akahane
Feb 1, 1992·The International Journal of Biochemistry·A D RichardsP A Charlton
Mar 1, 1983·Analytical Biochemistry·C Deyrup, B M Dunn
Sep 1, 2009·Biochimica Et Biophysica Acta·Derek R DeeRickey Y Yada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.