PMID: 7521736Jan 1, 1993Paper

Inhibition of PHA induced mononuclear cell proliferation by FK506 in combination with cyclosporine, methylprednisolone, 6-mercaptopurine and mycophenolic acid

Transplant Immunology
M HibbinsJ R Chapman

Abstract

In vitro inhibition of human peripheral blood mononuclear cell (PBMC) proliferation by immunosuppressive drugs has been shown to correlate with clinical outcomes in kidney transplant patients. The aim of our study was to analyse the degree of variability of in vitro interactions of FK506 (FK) with combinations of cyclosporin A (CyA), methylprednisolone (MP), 6-mercaptopurine (6ME), and mycophenolic acid (MPA) using phytohaemagglutinin (PHA) stimulated PBMCs. Our hypotheses were: that a wide range of interindividual variation would be detected; and that certain combinations of drugs would have a synergistic inhibitory effect on PHA stimulated PBMCs. Cells were cultured in supplemented RPMI 1640 for 65 hours at 37 degrees C in humidified 5% CO2 - 95% air and proliferative responses measured by 3H-thymidine incorporation over a further 24 hours. Inhibition of PBMC proliferation was assessed over 10 FK concentrations ranging from 1 x 10(-8) to 10 micrograms/ml using both volunteer controls (n = 51) and dialysis patients (n = 23). A wide range of interindividual variability of FK inhibition occurred throughout the range of FK concentrations tested, becoming less variable at the higher concentrations. The interactions of FK with CyA,...Continue Reading

References

Feb 1, 1992·Transplantation·H W SollingerR S Kauffman
Jul 1, 1991·Transplantation·A VathsalaB D Kahan
Jan 1, 1991·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·H JiangT Sonoda
Jun 1, 1989·Canadian Journal of Physiology and Pharmacology·B L Pope
Apr 1, 1987·Pharmacology & Toxicology·E LanghoffJ Ladefoged

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