PMID: 2140696Mar 20, 1990

Inhibition of phosphoglucomutase by vanadate

Biochemistry
M D PercivalM J Gresser

Abstract

Phosphoglucomutase is inhibited by a complex formed from alpha-D-glucose 1-phosphate (Glc-1-P) and inorganic vanadate (Vi). Both the inhibition at steady state and the rate of approach to steady state are dependent on the concentrations of both Glc-1-P and Vi. Inhibition is competitive versus alpha-D-glucose 1,6-bisphosphate (Glc-P2) and is ascribed to binding of the 6-vanadate ester of Glc-1-P (V-6-Glc-1-P) to the dephospho form of phosphoglucomutase (E). The inhibition constant for V-6-Glc-1-P at pH 7.4 was determined from steady-state kinetic measurements to be 2 x 10(-12) M. The first-order rate constant for approach to steady state increases hyperbolically with inhibitor concentration. The results are consistent with rapid equilibrium binding of V-6-Glc-1-P to E, with dissociation constant 1 x 10(-9) M, followed by rate-limiting conversion of the E.V-6-Glc-1-P complex to another species, E*.V-6-Glc-1-P, with first-order rate constant 4 x 10(-2)s-1. The rate constant determined for the reverse reaction, conversion of E*.V-6-Glc-1-P to E.V-6-Glc-1-P, is 2.5 x 10(-4)s-1. Formation of E*.V-6-Glc-1-P can also occur via binding of glucose 6-vanadate to the phospho form of phosphoglucomutase (E-P) followed by phosphoryl transfer ...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P P Layne, V A Najjar
Jul 1, 1971·Canadian Journal of Microbiology·E J LaishleyH D Peck
Jan 1, 1980·Annual Review of Biochemistry·J R Knowles
Jul 30, 1981·Biochemical and Biophysical Research Communications·F ClimentJ Carreras
Oct 15, 1983·Archives of Biochemistry and Biophysics·P NinfaliG Fornaini
Oct 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·W W ACKERMANN, V R POTTER

Citations

Dec 6, 1995·Molecular and Cellular Biochemistry·D C CransA D Keramidas
Jun 1, 1992·The International Journal of Biochemistry·T C WoonD P Fairlie
Jan 1, 1992·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·D Rehder
Mar 2, 1999·Protein Expression and Purification·K I SkoreyM J Gresser

Related Concepts

glucose-1-phosphate, sodium salt, (D-Glc)-isomer
Glucosephosphates
Leuconostoc
Phosphoglucomutase
Vanadyl
Glucose-6-Phosphate

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