Inhibition of phospholipid and platelet-dependent prothrombinase activity in the plasma of patients with lupus anticoagulants

British Journal of Haematology
M GalliC H Hemker

Abstract

Prothrombinase activity was analysed in the plasma of a series of patients with lupus anticoagulants (LAC). In the presence of purified PS-PC (20-80%) vesicles the prothrombinase activity triggered by kaolin was retarded by 2-3 min with respect with normal plasma. The maximal values of prothrombinase activity increased by increasing the amount of phospholipid vesicles. However, in the plasma of the patients they were always lower than those of normal plasma at each phospholipid concentration. Platelet-dependent prothrombinase activity was subsequently investigated. Again, both a delay in appearance and reduced peak values of prothrombinase activity were observed in the plasma of the patients. This inhibition was partially overcome by the addition of an excess of purified phospholipids. Finally, the effect of LAC IgG on platelet rich plasma-dependent prothrombinase activity was investigated. The main effect observed was a delay of the peak time of prothrombinase activity, while the maximal peaks were affected only by one IgG preparation. We conclude that LAC antibodies can react with both purified negatively-charged phospholipids and platelet procoagulant phospholipids and inhibit prothrombinase activity in a similar way in both...Continue Reading

References

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Citations

Jan 1, 1992·Journal of Clinical Immunology·L R SammaritanoM D Lockshin
Nov 1, 1995·Journal of Clinical Immunology·D B Cines, K R McCrae
Dec 10, 1999·Thrombosis Research·R LuddingtonT Baglin
Sep 1, 1993·British Journal of Haematology·S S PierangeliW L Dean
Jul 1, 1994·British Journal of Haematology·G H GoldsmithE N Harris
Apr 28, 1999·The Journal of Laboratory and Clinical Medicine·T L Ortel
Feb 15, 2000·Clinical and Laboratory Haematology·O AyoubN A Chaudhri
Oct 1, 1996·Lupus·E N Harris, S S Pierangeli
Oct 1, 1990·Seminars in Arthritis and Rheumatism·L R SammaritanoM D Lockshin

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