Inhibition of PKC-Induced COX-2 and IL-8 Expression in Human Breast Cancer Cells by Glucosamine

Journal of Cellular Physiology
Wan-Yu ChouYuh-Lin Wu

Abstract

Breast cancer is a common cancer leading to many deaths among females. Cyclooxygenase-2 (COX-2) and interleukin-8 (IL-8) are two highly expressed inflammatory mediators to be induced by the protein kinase C (PKC) signaling via various inflammatory stimuli and both contribute significantly to cancer metastasis/progression. Glucosamine has been shown to act as an anti-inflammation molecule. The aim of this study was to clarify the role and acting mechanism of glucosamine during the PKC-regulation of COX-2/IL-8 expression and the associated impact on breast cancer. In MCF-7 breast cancer cells, glucosamine effectively suppresses the PKC induction of COX-2 and IL-8 promoter activity, mRNA and protein levels, as well as the production of prostaglandin E(2) (PGE(2)) and IL-8. Glucosamine is able to promote COX-2 protein degradation in a calpain-dependent manner and IL-8 protein degradation in calpain-dependent and proteasome-dependent manners. The MAPK and NF-κB pathways are involved in PKC-induced COX-2 expression, but only the NF-κB pathway is involved in PKC-induced IL-8 expression. Glucosamine attenuates PKC-mediated IκBα phosphorylation, nuclear NF-κB translocation, and NF-κB reporter activation. Both PGE(2) and IL-8 promote cel...Continue Reading

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Citations

Aug 23, 2016·International Journal of Molecular Sciences·Yi-Ning ChaoYuh-Lin Wu
Oct 21, 2018·Journal of Cellular Physiology·Nasser Hashemi GoradelKeywan Mortezaee
Oct 30, 2018·Journal of Cellular Physiology·Jiaxin ChenXiaotong Hu
Aug 30, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Do Luong HuynhDong Kee Jeong
Sep 20, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fatemeh ZahedipourKhadijeh Jamialahmadi
Mar 25, 2021·BMC Research Notes·Mohadeseh Zarei-GhobadiSayed-Hamidreza Mozhgani

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