Inhibition of PKCgamma membrane translocation mediated morphine preconditioning-induced neuroprotection against oxygen-glucose deprivation in the hippocampus slices of mice

Neuroscience Letters
Ya LiuBingxi Zhang

Abstract

We previously reported that novel protein kinase C (nPKC) epsilon and N-methyl-d-aspartic acid (NMDA) receptors participated in morphine preconditioning (MP)-induced neuroprotection. In this study, we used Western blot analysis, 2,3,5-triphenyltetrazolium chloride (TTC) staining and lactate dehydrogenase (LDH) leakage assay to determine the involvement of conventional PKC isoforms (cPKC) in MP-induced neuroprotection against oxygen-glucose deprivation (OGD). Hippocampus slices (400-microm thickness) from healthy male BALB/c mice exposed to OGD for 5-45 min to mimic mild, moderate and severe ischemia in the presence of MP pretreatment. We found that OGD-induced damage in neuronal cell survival rate and LDH leakage could be improved by MP pretreatment (3 microM) within 20 min of OGD, which was abolished by concomitant incubation with non-selective opioid receptor antagonist naloxone (Nal, 50 microM). The results of Western blot analysis showed that only cPKCgamma membrane translocation, not alpha, betaI and betaII, increased under the condition of OGD 10 min and 2h reperfusion (OGD/2h), and this increment of cPKCgamma membrane translocation was inhibited by MP pretreatment. To further elucidate the role of cPKCgamma in MP-induced...Continue Reading

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Citations

Aug 23, 2011·The Journal of Pharmacology and Experimental Therapeutics·Li YangThomas J Abbruscato
Oct 2, 2009·Peptides·Richard J Bodnar
Jan 1, 2015·Cell Death Discovery·Barbara MainoSebastiano Cavallaro
Jul 25, 2019·International Journal of Molecular Sciences·Donatella PastoreDavid Della-Morte
Aug 11, 2021·Experimental and Therapeutic Medicine·Xiao-Yan ZhaoGu-Yan Wang

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