PMID: 2505852Sep 11, 1989Paper

Inhibition of platelet-activating factor biosynthesis via the acetyltransferase by arachidonic and oleic acids in ionophore A23187-stimulated bovine neutrophils

Biochimica Et Biophysica Acta
E RemyJ Remacle

Abstract

Platelet-activating factor (PAF) is a phospholipid mediator of inflammation and allergy that is synthesized by several inflammatory cells including neutrophils. Addition of exogenous arachidonic acid to ionophore A23187-stimulated bovine neutrophils led to the inhibition of PAF biosynthesis assayed by incorporation of [3H]acetate into PAF and by bioassay; under the same conditions, leukotriene B4 (LTB4) formation was not decreased. The activities of the PAF metabolism enzymes indicated that the PAF synthesis inhibition by arachidonic acid is mediated via the acetyltransferase inhibition which is the last enzyme of the PAF formation. Another unsaturated fatty acid, oleic acid, exhibited the same inhibitory effect on [3H]acetate-PAF formation; however, the saturated stearic acid did not lead to any inhibition. These findings suggest that liberation of unsaturated fatty acids from membrane phospholipids, as a consequence of phospholipase A2 activation, would modulate PAF formation via inhibition of the acetyltransferase. In addition, the utilization of arachidonic acid oleic acids in activated neutrophils furnishes an easy means of blocking PAF synthesis in order to understand the role of this mediator in cellular processes.

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Citations

Apr 29, 1996·European Journal of Pharmacology·H NarabaS Oh-ishi
Jan 1, 1996·Journal of Lipid Mediators and Cell Signalling·J SugataniS Ito
Mar 19, 2014·European Journal of Nutrition·P DetopoulouS Antonopoulou
Aug 19, 2007·Journal of Lipid Research·Jiawei ChenThomas M McIntyre
Dec 30, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·E FragopoulouS Antonopoulou

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