Inhibition of platelet migration. A simple technic for typing of PLA1 and the detection of platelet allo- and autoantibodies

Revue française de transfusion et immuno-hématologie
M F Reznikoff-Etievant, M Larsen

Abstract

In vitro, platelets can migrate in an active way. This migration can be immunologically inhibited through specific antibodies. This test (PMI-test) can be easily carried out in any laboratory. In that study this test was used: 1) For the detection of anti-platelet allo-antibodies in neonatal thrombopenia. Out of nine cases studied, two anti-PLA1 were identified and three anti-HLA antibodies (anti-B8, anti-B17+21 and a polyspecific serum) detected thanks to the micro-lymphocyto-toxicity test were confirmed by that test. 2) To find free auto-antibodies in the sera of patients suffering from idiopathic thrombopenic purpura. In five cases out of twelve the presence of auto-antibodies could be shown, that is 41,6%. In some cases, the auto-antibodies showed a certain degree of specificity as it had already been pointed out in other studies. When the thrombopenias were associated with other diseases, all the tests were negative. 3) For the PLA1 typing of donors: 1,85% donors submitted to the test proved to be PLA1 negative. 4) To identify anti-HLA antibodies and to compare this test with the micro-lymphocyto-toxicity-test: the anti-A2, A11, B8, B17, CW3 and CW4 antibodies used titrated the same in both tests. 5) Eventually to study th...Continue Reading

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