Inhibition of pre-mRNA splicing promotes root hair development in Arabidopsis thaliana

Plant & Cell Physiology
Miku IshizawaKumi Sato-Nara

Abstract

Root hairs protruding from epidermal cells increase the surface area for water and nutrient uptake. Various environmental factors, including light, promote root hair formation in Arabidopsis thaliana. Light regulates the expression of a large number of genes at the transcriptional and post-transcriptional level; however, there is little information linking the light response to root hair development. Here, we describe a novel mutant, light-sensitive root-hair development 1 (lrh1), that displays enhanced root hair development in response to light. Hypocotyl and root elongation was inhibited in the lrh1 mutant, which had a late flowering phenotype. We identified the gene encoding the p14 protein, a putative component of the SF3b complex essential for pre-mRNA splicing, as being responsible for the lrh1 phenotype. Indeed, regulation of alternative splicing was affected in lrh1 mutants and splicing inhibitor treatment mimicked the lrh1 phenotype. Genome-wide alterations of splicing including differential splicing events of light signaling- and clock-related genes were found in lrh1 as well as a difference in transcriptional regulation of multiple genes including up-regulation of essential genes for root hair development. These resu...Continue Reading

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Sep 11, 2019·Plant & Cell Physiology·Misato OhtaniMartin Crespi

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