Inhibition of progesterone secretion from granulosa cells by estradiol and androgens in the domestic hen

Endocrinology
P A JohnsonJ M Bahr

Abstract

We previously reported no difference in progesterone (P4) secretion from the granulosa layer of the largest follicle (F1) of the domestic hen regardless of the maturity of the F1 follicle. However, coincubation of the granulosa and thecal layers resulted in inhibition of P4 secretion from the less mature F1, but not from the more mature F1. The goal of this study was to determine if estradiol (E2) and androgens secreted by the thecal layer suppress P4 production by the granulosa cells. We removed the granulosa layer from less mature F1 follicles and dispersed granulosa cells (1 x 10(5)) were incubated (3 h) in triplicate with one of these treatments: control, E2, testosterone (T), androstenedione (A), and dihydrotestosterone (DHT; at concentrations of 1 x 10(-7), 1 x 10(-6), and 1 x 10(-5) M), LH (100 ng) as well as LH plus E2, T, A, and DHT at the same concentrations. P4 secretion was measured in the medium and cells, and the experiment was replicated seven times. We found a dose-related suppression of basal and LH-stimulated P4 production by all steroids. In a second experiment (n = 3-5), we tested the specificity of the androgens in suppressing P4 production by granulosa cells by using the aromatase inhibitor 7-(4'-amino)phe...Continue Reading

Citations

Jan 1, 1990·The Journal of Experimental Zoology. Supplement : Published Under Auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry·J Bahr
Aug 28, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·P L RangelC G Gutierrez
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·P L RangelC G Gutierrez
Oct 6, 2000·General and Comparative Endocrinology·A J DavisP A Johnson
Mar 8, 2016·Asian-Australasian Journal of Animal Sciences·R E Caicedo RivasM Kamiyoshi
Nov 25, 2003·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·A SechmanH Paczoska-Eliasiewicz
Apr 11, 2014·General and Comparative Endocrinology·P L Rangel, C G Gutierrez
Feb 16, 2017·Annual Review of Animal Biosciences·Janice M Bahr

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