Inhibition of proliferation and induction of apoptosis in human breast cancer cells by lauryl gallate

Carcinogenesis
Annarica CalcabriniJorge Martín-Pérez

Abstract

Lauryl gallate is an antioxidant food additive showing low toxicity to normal cells. Here, its antiproliferative effect has been studied on three human breast cancer cell lines: estrogen-dependent, wild-type p53, MCF7; estrogen-independent, non-functional p53, MDA-MB-231 and MCF7 ADR, which overexpresses P-glycoprotein (P-gp) and displays a multidrug-resistant phenotype. Lauryl gallate inhibited proliferation and induced cell cycle alterations in all three cell lines without altering P-gp functionality in the drug-resistant cells. A stable arrest in G(1) phase was observed in MCF7, while a slow-down of cell cycle progression was induced in the other two cell lines. Lauryl gallate increased p53 expression only in MCF7, and upregulated p21(Cip1) and reduced cyclin D1 levels in all three cell lines. The induction of apoptosis, demonstrated by annexin V-FITC labeling, PARP cleavage and mitochondrial membrane depolarization and morphological alterations, were clearly detected in MCF7 ADR and MDA-MB-231 and to a minor extent in MCF7. Overexpression of Bcl-2 in MCF7 ADR cells demonstrated its protective role against morphological alterations and apoptosis. Lauryl gallate induction of p21(Cip1) and apoptosis observed in all three cell ...Continue Reading

Citations

May 27, 2009·International Journal of Food Sciences and Nutrition·Pablo García-SolísMauricio Díaz-Muñoz
Jul 23, 2011·Drug and Chemical Toxicology·Soumaya Kilani-JaziriXavier Ronot
Mar 15, 2016·Oncogene·T LeeJ Pelletier
Dec 13, 2016·Cellular Signalling·David CerezoElena Martín-Orozco
May 6, 2008·Apoptosis : an International Journal on Programmed Cell Death·Lakshmishri LahiryTanya Das
Jun 23, 2015·Molecular Reproduction and Development·Nikola KolundžićLjiljana Vićovac
Jul 10, 2013·International Journal of Oncology·Jingjing ZhuAlphons Vermorken
Jun 9, 2009·British Journal of Haematology·Tae Young KimUNKNOWN Korean Multiple Myeloma Working Party (KMMWP)
Apr 12, 2020·Cancers·Alessia Roma, Paul A Spagnuolo
Apr 30, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Małgorzata JurakSantiago Martín
Dec 26, 2006·Biochimica Et Biophysica Acta·Jean-Claude ChambardPhilippe Lenormand

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis