Inhibition of protein kinase C by H-7 potentiates the release of oleic, linoleic and arachidonic acids in A23187-stimulated human neutrophils

Biochemical and Biophysical Research Communications
J Conquer, V G Mahadevappa

Abstract

This present report describes the effect of H-7, a protein kinase C inhibitor, on the release of oleic, linoleic and arachidonic acids in A23187-stimulated neutrophils. Surprisingly, the inhibitor potentiated the release of all three unsaturated fatty acids in neutrophils stimulated with A23187 alone. In contrast, released oleic acid, linoleic acid and arachidonic acid in phorbol 12-myristate 13-acetate-primed neutrophils were attenuated by 35, 47 and 33%, respectively, in the presence of H-7 (300 microM). Phorbol 12-myristate 13-acetate (PMA) had no effect on A23187-stimulated release of saturated fatty acids. Both PMA and H-7 when used alone had no effect on the release of saturated or unsaturated fatty acids. We, therefore, conclude that H-7 may have effects other than inhibiting PMA-primed responses including superoxide generation, degranulation and arachidonic acid release in human neutrophils.

References

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Citations

Jul 1, 1991·The Journal of Membrane Biology·Y SuzukiK Sato
Sep 1, 1992·The International Journal of Biochemistry·E W Haeffner, J B Strosznajder
Mar 1, 1997·Journal of Lipid Mediators and Cell Signalling·A Siafaka-KapadaiM A Javors
Dec 1, 1992·Chemico-biological Interactions·A Van der Vliet, A Bast

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