Abstract
We have evaluated the protein kinase C (PKC) activity in two series of cultured cell lines presenting the multidrug-resistance (MDR) phenotype and in the corresponding wild-type cells: the human KB 3.1, KB A1 and KB 8.5 cell lines, and the rat C6, C6 0.5 and C6 1V cell lines. We have observed an increase in PKC activity in the MDR cell lines of the KB cell lineage, proportional to their degree of resistance to doxorubicin. In contrast, the MDR cell lines of the C6 cell lineage presented no change (C6 0.5) or even decrease (C6 1V) in PKC activity; the basal level of PKC activity in C6 cells was, however, 50-fold higher than in KB 3.1 cells. We have tested, in these lines, the effect of four modulators of MDR: verapamil, cyclosporin A, quinine and S-9788, on doxorubicin acytotoxicity and on PKC activity. We observed that cyclosporin A and S-9788, which were the most active on MDR reversal, were able to inhibit PKC activity in the KB resistant lines as well as in all C6 lines, whereas verapamil and quinine had only marginal effects on PKC activity. The distribution of PKC isoenzymes was studied by Western blots. The PKC alpha, gamma and delta isoforms were increased in the KB resistant lines as compared to wild-type cells, which c...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Apr 1, 1992·British Journal of Cancer·S HuetJ Robert
Oct 23, 1992·Science·Y Nishizuka
Jun 1, 1991·Cancer Communications·G YuR I Glazer
Jan 1, 1991·Pharmacology & Therapeutics·S Stabel, P J Parker
Jan 2, 1991·International Journal of Cancer. Journal International Du Cancer·C LanziF Zunino
Jul 4, 1991·Cancer Letters·N E Ward, C A O'Brian
May 31, 1990·Biochemical and Biophysical Research Communications·T C ChambersG Eilon
Dec 31, 1990·Biochemical and Biophysical Research Communications·W SatoT Tsuruo
Nov 30, 1990·Biochemical and Biophysical Research Communications·B SparatoreS Pontremoli
Jan 1, 1990·Advances in Pharmacology·E GeorgesV Ling
Jan 30, 1990·Biochemical and Biophysical Research Communications·A AquinoR I Glazer
Feb 15, 1988·International Journal of Cancer. Journal International Du Cancer·S Huet, J Robert
Jan 1, 1989·Annual Review of Biochemistry·J A Endicott, V Ling
Mar 27, 1989·FEBS Letters·C A O'BrianI J Fidler
Mar 1, 1988·British Journal of Cancer·P R Twentyman
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L FineB A Chabner
May 2, 1986·Science·D W ShenM M Gottesman
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K UedaI Pastan
Oct 29, 1987·Biochemical and Biophysical Research Communications·S T PalayoorW N Hait
Aug 15, 1970·Nature·U K Laemmli
Jan 1, 1984·Analytical Biochemistry·M S BlakeE C Gotschlich
Jul 17, 1995·Biochemical Pharmacology·Y P Hu, J Robert
Sep 7, 1993·Biochemistry·S E BatesA T Fojo
Mar 1, 1994·International Journal of Cancer. Journal International Du Cancer·C MullerG Laurent
Jan 1, 1993·The Journal of Pharmacy and Pharmacology·K MiyamotoM Koyama
Jan 1, 1993·Journal of Cancer Research and Clinical Oncology·B SchottJ Robert
Aug 19, 1993·International Journal of Cancer. Journal International Du Cancer·B SchottJ Robert
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Sep 1, 1993·Pharmacology & Therapeutics·A Basu
Jan 1, 1993·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S HuetJ Robert
Oct 11, 1993·FEBS Letters·C C Chen
Jan 19, 1996·The Journal of Biological Chemistry·U A GermannM M Gottesman
Jun 7, 1996·The Journal of Biological Chemistry·H R GoodfellowC F Higgins
Citations
Jul 18, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·M BarancíkA Breier
Dec 4, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D Del BufaloG Zupi
Nov 13, 2008·Cytotechnology·M G RumsbyJ R Warr
Sep 23, 2009·Photochemistry and Photobiology·Jay E WolvertonJeffrey B Travers
Jan 25, 2005·Nephron. Experimental Nephrology·Clarissa R NascimentoMarcia A M Capella
Feb 20, 2014·Peptides·Ana González-OrtegaMiguel Muñoz
Mar 1, 2012·Biomaterials·Yan LiYen Wei
Jun 15, 2010·Peptides·Miguel MuñozRafael Coveñas
May 13, 2014·The International Journal of Biochemistry & Cell Biology·Tsai-Hsien HungKowit Yu Chong
Jun 5, 2014·Nutrition and Cancer·Harshita ChaudharySriram Seshadri
Oct 4, 2006·Physiological Reviews·Balázs SarkadiAndrás Váradi
Aug 11, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Phillip KantharidisJohn Zalcberg