PMID: 7542047Jan 1, 1995Paper

Inhibition of protein-tyrosine kinase activity in intact cells by the aptameric action of oligodeoxynucleotides

Antisense Research and Development
R C BerganL Neckers

Abstract

Direct interaction of oligodeoxynucleotides (ODNs) with proteins represents one of the nonantisense-mediated effects of ODNs. Phosphorothioate-capped ODNs have been shown to inhibit directly the in vitro kinase activity of the chronic myelogenous leukemia-associated protein-tyrosine kinase p210bcr-abl. In this study we have determined the efficacy of this aptameric ODN in a cellular system using the K562 chronic myelogenous leukemia-derived cell line. Significant effects upon cellular phosphotyrosine content, as well as cellular growth in soft agar, are observed. These effects are sequence specific and are not mediated through changes in p210bcr-abl protein levels. Additional ODNs are described that also reduce cellular phosphotyrosine levels and inhibit growth in soft agar but do not inhibit p210bcr-abl kinase activity in vitro.

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Citations

Jan 1, 1996·Antisense & Nucleic Acid Drug Development·T F SmetsersE J Mensink
Jun 1, 1997·Antisense & Nucleic Acid Drug Development·R C Bergan
Mar 22, 2001·Antisense & Nucleic Acid Drug Development·Y Liu, R Bergan
Jan 1, 1996·Antisense & Nucleic Acid Drug Development·G HartmannS Endres
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·I V Lebedeva, C A Stein
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Sep 1, 1996·Cancer Metastasis Reviews·I Gibson
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Oct 18, 2000·Advanced Drug Delivery Reviews·S Dokka, Y Rojanasakul

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