Inhibition of proteoglycan synthesis induces an increase in follicle stimulating hormone (FSH)-stimulated estradiol production by immature rat Sertoli cells

Molecular and Cellular Endocrinology
N T PhamantuJ Bocquet

Abstract

In order to define the possible involvement of proteoglycans (PG) in the regulation of Sertoli cell functions, we have examined the effect of para-nitrophenyl-beta-D-xyloside (PNPX), a specific inhibitor of PG synthesis, on follicle stimulating hormone (FSH)-dependent estradiol production by immature rat Sertoli cells. Addition of PNPX to the culture medium induced a dose-dependent inhibition of 35S-labeled PG synthesis in Sertoli cells both in the medium and the cell layer. Simultaneously there was a drastic increase in 35S-labeled secreted glycosaminoglycans. By 1 mM PNPX, syntheses of chondroitin sulfate proteoglycans released into culture medium and of heparan sulfate proteoglycans associated with the cell layer were 35% of values from untreated cells. Simultaneously, PNPX induced a twofold (mean of seven experiments, range 17-250%) enhancement of FSH (100 ng/ml)-stimulated estradiol production. In each individual experiment, there was an inverse relationship between the amplitude of PNPX-induced increase in FSH responsiveness and the FSH capability to stimulate basal estradiol production in cultured rat Sertoli cells. The effect of PNPX on FSH-stimulated aromatase activity was not mimicked by para-nitrophenyl-beta-D-galact...Continue Reading

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Citations

Apr 19, 1996·Molecular and Cellular Endocrinology·J R McFarlaneG P Risbridger
Nov 26, 1999·Biochimica Et Biophysica Acta·G FagnenP J Bonnamy
Jun 16, 1999·The Journal of Steroid Biochemistry and Molecular Biology·N GrudetS Carreau
Aug 12, 1999·International Journal of Andrology·S CarreauJ Levallet
Dec 23, 2008·Biochemical and Biophysical Research Communications·Yvonne M Bastiaansen-JenniskensGerjo J V M VanOsch
Aug 13, 2003·Cell Transplantation·Dwaine F EmerichCraig R Halberstadt

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