Inhibition of Pseudomonas aeruginosa biofilm formation on wound dressings

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Kenneth S BrandenburgCharles J Czuprynski

Abstract

Chronic nonhealing skin wounds often contain bacterial biofilms that prevent normal wound healing and closure and present challenges to the use of conventional wound dressings. We investigated inhibition of Pseudomonas aeruginosa biofilm formation, a common pathogen of chronic skin wounds, on a commercially available biological wound dressing. Building on prior reports, we examined whether the amino acid tryptophan would inhibit P. aeruginosa biofilm formation on the three-dimensional surface of the biological dressing. Bacterial biomass and biofilm polysaccharides were quantified using crystal violet staining or an enzyme linked lectin, respectively. Bacterial cells and biofilm matrix adherent to the wound dressing were visualized through scanning electron microscopy. D-/L-tryptophan inhibited P. aeruginosa biofilm formation on the wound dressing in a dose dependent manner and was not directly cytotoxic to immortalized human keratinocytes although there was some reduction in cellular metabolism or enzymatic activity. More importantly, D-/L-tryptophan did not impair wound healing in a splinted skin wound murine model. Furthermore, wound closure was improved when D-/L-tryptophan treated wound dressing with P. aeruginosa biofilms...Continue Reading

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Citations

Sep 29, 2017·The Journal of Microbiology·Xi-Hui Li, Joon-Hee Lee
Aug 19, 2017·Scientific Reports·Xi-Hui LiJoon-Hee Lee
Dec 5, 2019·Scientific Reports·Jing LuElizabeth J Harry
Jun 20, 2019·Frontiers in Cellular and Infection Microbiology·Manon Ruffin, Emmanuelle Brochiero
Dec 17, 2020·Biomedical Materials·Günnur OnakOzan Karaman
Jun 10, 2021·Journal of Tissue Viability·Jennifer K ZindleKath M Bogie

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