Inhibition of rat platelet aggregation by Urtica dioica leaves extracts

Phytotherapy Research : PTR
Mohammed El HaouariH Mekhfi

Abstract

Platelet hyperactivity plays an important role in arterial thrombosis and atherosclerosis. The present study was undertaken to investigate the effects of different extracts of Urtica dioica leaves on platelet aggregation. Rat platelets were prepared and incubated in vitro with different concentrations of the tested extracts and aggregation was induced by different agonists including thrombin (0.5 U/mL), ADP (10 microm), epinephrine (100 microm) and collagen (5 mg/mL). The crude aqueous extract inhibited thrombin-induced platelet aggregation in a dose-dependent manner. At 1 mg/mL, the percent inhibition was 17.1 +/- 4.2%. Soxhlet extraction of the plant leaves with different successive solvents showed that the ethyl acetate extract exhibited the most antiaggregant effect with an inhibition of 76.8 +/- 6.1% at 1 mg/mL. Flavonoids isolated from the plant leaves, produced a strong inhibitory effect on thrombin-induced platelet aggregation with an IC(50) of 0.25 +/- 0.05 and 0.40 +/- 0.04 mg/mL for genins and heterosidic flavonoids, respectively. Flavonoids also markedly inhibited platelet aggregation induced by ADP, collagen and epinephrine. It is concluded that Urtica dioica has an antiplatelet action in which flavonoids are mainl...Continue Reading

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Citations

Aug 25, 2011·Journal of Medicinal Food·Zeynep GencBetul Demirci
Dec 22, 2015·Molecular and Cellular Biochemistry·Joanna Kolodziejczyk-CzepasAnna Stochmal
Sep 15, 2007·Journal of Ethnopharmacology·Leila Shahriyary, Razieh Yazdanparast
Aug 8, 2007·Journal of Ethnopharmacology·Mohammed El HaouariGinés M Salido
May 12, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Julia E ChrubasikSigrun A Chrubasik
Oct 27, 2009·Phytotherapy Research : PTR·Melainie CameronSigrun Chrubasik
Apr 12, 2016·Phytotherapy Research : PTR·Mohammed El Haouari, Juan A Rosado
Nov 2, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Sadegh FattahiHaleh Akhavan-Niaki
Nov 10, 2013·Journal of Ethnopharmacology·Taiana Magalhães PierdonáLuzia Kalyne Almeida Moreira Leal
Feb 24, 2015·Life Sciences·Olta Allkanjari, Annabella Vitalone
Dec 17, 2009·Phytotherapy Research : PTR·Masoud Shabani DomolaMichael B Wheeler
Sep 25, 2018·Mini Reviews in Medicinal Chemistry·Mohammed El Haouari, Juan A Rosado
Dec 14, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Natalia MarcinczykEwa Chabielska
Jan 14, 2020·PLoS Neglected Tropical Diseases·Alireza BadirzadehAlireza Salimi Chirani
Jun 5, 2019·Progress in Biophysics and Molecular Biology·Raouia DhouibiKamilia Ksouda
Sep 10, 2008·Journal of Agricultural and Food Chemistry·Patrizia PinelliAnnalisa Romani

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