Inhibition of responder cell activity in mixed leukocyte culture reactions. I. Evidence of cross-reactivity of B7-antibody with HLA-B8

Tissue Antigens
E de Rooij-DoyerJ J van Rood

Abstract

In a previous report we described a human allo-antiserum which showed MHC restricted dual specific inhibition of mixed leukocyte culture (MLC) reactions (de Rooij et al. 1980). Responder cells were preincubated with the antiserum and washed. Inhibition of MLC reactions occurred if HLA-B8 positive responder cells and HLA-B7 or B40 positive stimulator cells were used. The mechanism of inhibition by this antiserum was investigated and described in this report. The results strongly suggested that the observed inhibition was due to HLA-B7 specific antibody molecules cross-reacting with HLA-B8 antigens. We decided on the following mechanism: HLA-B7 specific antibody molecules bind to HLA-B8 antigens on responder cells at 4 degrees C. Bound antibody molecules area readily released at 37 degrees C and the bind preferentially to HLA-B7 or -B40 positive stimulator cells. Stimulator cells are then lysed by antibody dependent cellular cytotoxicity (ADCC), resulting in impaired MLC reaction. This conclusion was derived from the following observations: 1. HLA-B7 positive cells could be lysed due to antibody molecules bound to B8 positive cells: a. Cells coated with antibody molecules lysed B7 or B40 positive target cells. b. Antibody molecul...Continue Reading

References

Jan 1, 1977·Scandinavian Journal of Immunology·J J van RoodA Termijtelen
Mar 1, 1978·Tissue Antigens·M Jonker, J J van Rood
Jan 1, 1980·Journal of Immunological Methods·J W BruningR Arentzen
Sep 1, 1975·European Journal of Immunology·H Binz, B A Askonas
Jan 1, 1980·Tissue Antigens·M Jonker, L de Rooy-Doyer
Jan 1, 1946·Journal of the American Chemical Society·M L PETERMANN

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