Inhibition of Rho‑associated protein kinase increases the ratio of formation of blastocysts from single human blastomeres

Molecular Medicine Reports
Sunxing HuangCanquan Zhou

Abstract

Y‑27632 is a specific inhibitor of Rho‑associated protein kinases (ROCKs), which are downstream effectors of Rho GTPase. The present study aimed to determine the effect of the specific ROCK inhibitor, Y‑27632, on fresh human embryos and on single blastomeres obtained from discarded human embryos. A total of 784 poor‑quality embryos were included, of which 526 were allocated to blastocyst culture directly and the remaining 258 were allocated to blastomere isolation. Embryos and single blastomeres were cultured either with, or without, Y‑27632. Embryonic development was observed and recorded daily from day 5 onwards. Y‑27632 did not affect the ratio of blastocyst formation or the quality of the human embryos. The duration of blastocyst formation was compared between the two groups in the embryo culture. On day 5, the blastocyst formation ratio in the experimental group was 11.4% (26/228), which was significantly (P=0.015) lower than the corresponding rate (19.7%; 44/223) in the control group. Survival analysis of the blastocyst formation duration showed that the median formation duration in the experimental group was significantly higher than that of the control group. The present study also obtained 1,192 blastomeres from 258 di...Continue Reading

References

Jan 26, 1999·Developmental Biology·L ClaytonM H Johnson
Jun 5, 2004·Biochemical and Biophysical Research Communications·Rikako KawagishiYuji Murata
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Jul 31, 2007·Lancet·Alastair G Sutcliffe, Michael Ludwig
Dec 25, 2007·Molecular and Cellular Endocrinology·Nicolas DardSophie Louvet-Vallée
Jul 28, 2009·Human Reproduction·Mieke GeensHilde Van de Velde
Nov 20, 2009·Anatomia, histologia, embryologia·E GumusC Kaloglu
Aug 10, 2010·Biochemical and Biophysical Research Communications·Seiichi KoikeMasayuki Masu
Dec 20, 2011·In Vitro Cellular & Developmental Biology. Animal·Peng ZhangXiangyun Li
Feb 7, 2013·Human Reproduction Update·E MantikouS Mastenbroek
Aug 16, 2013·Biology of Reproduction·Arlene May A LaenoVernadeth B Alarcon
Feb 25, 2014·Histochemistry and Cell Biology·Xing DuanShao-Chen Sun
Apr 1, 2014·Theriogenology·Yu ZhangShao-Chen Sun

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Citations

Jun 30, 2018·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Sulmaz KamalifarMohamad Reza Azarpira
Apr 30, 2020·Animals : an Open Access Journal From MDPI·Hammed A TukurIslam M Saadeldin
Jan 29, 2021·Frontiers in Cell and Developmental Biology·Islam M SaadeldinRamya A Sindi

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Methods Mentioned

BETA
GTPase
PCR

Software Mentioned

SPSS
Sequence Detector

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