PMID: 3771637Oct 1, 1986Paper

Inhibition of ribulose bisphosphate carboxylase assembly by antibody to a binding protein

The Journal of Cell Biology
S CannonH Roy

Abstract

We have developed an assay to monitor in vitro the posttranslational assembly of the chloroplast protein, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Most of the newly synthesized 55-kD catalytic ("large") subunits of this enzyme occur in a 29S complex together with 60- and 61-kD "binding" proteins. When the 29S complex is incubated with ATP and MgCl2 it dissociates into subunits, and the formerly bound large subunits now sediment at 7S (still faster than expected for a monomer). Upon incubation at 24 degrees C, these large subunits assemble into RuBisCO. The minority of newly made large subunits which are not bound to the 29S complex also sediment at 7S. When endogenous ATP was removed by addition of hexokinase and glucose, the dissociation of the 29S complex was inhibited. Nevertheless, the 7S large subunits assembled into RuBisCO, and did so to a greater extent than in controls retaining endogenous ATP. Thus the 7S large subunits are also assembly competent, at least when ATP is removed. Apparently, in chloroplast extracts, ATP can have a dual effect on the assembly of RuBisCO: on the one hand, even at low concentrations it can inhibit incorporation of 7S large subunits RuBisCO; on the other hand, at higher co...Continue Reading

Citations

Oct 1, 1996·Plant Molecular Biology·R S BostonE Vierling
Apr 1, 1988·Photosynthesis Research·R J Ellis, S M Van Der Vies
Nov 1, 1990·Photosynthesis Research·S M Newman, R A Cattolico
Jun 10, 2006·Photosynthesis Research·Boovaraghan BalajiHarry Roy
Jan 1, 1989·Electron Microscopy Reviews·A Holzenburg, F Mayer
Mar 13, 2012·Plant Science : an International Journal of Experimental Plant Biology·J H HaoQ M Shang
Dec 16, 1987·Biochemical and Biophysical Research Communications·K StrzalkaT Akazawa
Jul 28, 1995·The Journal of Biological Chemistry·P V ViitanenJ Soll
Apr 15, 2000·The Journal of Biological Chemistry·R DicksonP V Viitanen
Dec 2, 1988·Biochimica Et Biophysica Acta·H RoyM Gilson

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