PMID: 6987669Feb 1, 1980Paper

Inhibition of RNA polymerase activity by the Escherichia coli protein biosynthesis elongation factor Ts

Proceedings of the National Academy of Sciences of the United States of America
C K Biebricher, M Druminski

Abstract

The transcribing activity of DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) from Escherichia coli is inhibited in vitro by addition of preparations of elongation factor Ts purified to homogeneity. The inhibitory activity of elongation factor Ts on the RNA polymerase activity and the enzymatic activity of elongation factor Ts show the same temperature sensitivity. The extent of inhibition is strongly dependent on the template used for transcription. A mechanism for the control of RNA synthesis in vivo based on this inhibition found in vitro is proposed.

References

May 1, 1976·Cell·A J van OoyenP Jorgensen
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A V Furano
Jul 6, 1973·Nature·A Travers
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T BlumenthalK Weber
Nov 21, 1970·Nature·A TraversR F Schleif
Nov 1, 1970·Archives of Biochemistry and Biophysics·D L Miller, H Weissbach
Apr 24, 1972·European Journal of Biochemistry·H SchallerI Nietzschmann
Mar 21, 1967·Biochemical and Biophysical Research Communications·C Babinet

Related Concepts

Alkalescens-Dispar Group
Guanosine Pentaphosphate
Elongation Factor
Chemical Precipitation
Plasma Protein Binding Capacity
Transcriptase

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