Inhibition of ROCK signaling pathway accelerates enteric neural crest cell-based therapy after transplantation in a rat hypoganglionic model.

Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
Yu-Ying ZhaoYa Gao

Abstract

Hirschsprung's disease (HSCR) is a congenital gastrointestinal disorder, characterized by enteric ganglia absence in part or entire of the colon, due to abnormal colonization and migration of enteric neural crest cells (ENCCs) during development. Currently, besides surgery which is the main therapy for HSCR, the potential of stem cell-based transplantation was investigated as an alternative option. Although promising, it has limitations, including poor survival, differentiation, and migration of the grafted cells. We hypothesized that modulation of extracellular factors during transplantation could promote ENCCs proliferation and migration, leading to increased transplantation efficiency. Considering that the RhoA/ROCK pathway is highly involved in cytoskeletal dynamics and neurite growth, our study explored the effect of inhibition of this pathway to improve the success of ENCCs transplantation. Enteric neural crest cells were isolated from rat embryos and labeled with a GFP-tag. Cell viability, apoptosis, differentiation, and migration assays were performed with and without RhoA/ROCK inhibition. Labeled ENCCs were transplanted into the muscle layer of an induced hypoganglionic rat model followed by intraperitoneal injections ...Continue Reading

References

Apr 15, 2003·Molecular and Cellular Neurosciences·Jaimie F BorisoffWolfram Tetzlaff
Sep 19, 2003·The Journal of Surgical Research·Toru MurataMasayuki Imamura
Oct 7, 2004·Developmental Biology·Xiao Huang, Jean-Pierre Saint-Jeannet
Mar 3, 2005·European Journal of Pharmacology·Oğuz KökselKansu Büyükafşar
Aug 5, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M-A MicciP J Pasricha
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Aron B Jaffe, Alan Hall
Oct 10, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Maria-Adelaide Micci, Pankaj Jay Pasricha
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Jul 11, 2009·The Journal of Surgical Research·Min LiuWei J Zhang
Apr 3, 2010·Nature Protocols·Gabsang LeeLorenz Studer
Jan 27, 2011·British Journal of Pharmacology·O BayguinovS D Koh
Aug 16, 2011·The Journal of Clinical Investigation·Elly Sau-Wai NganPaul Kwong-Hang Tam
Feb 9, 2012·International Journal of Colorectal Disease·Cornelia Irene HaglKarl-Herbert Schäfer
Mar 7, 2012·Nature Reviews. Gastroenterology & Hepatology·John B Furness
Sep 19, 2012·Cell Structure and Function·Michiro IizukaAkira Mizoguchi
Oct 19, 2012·Cell Adhesion & Migration·Ying Zhang, Lee Niswander
Mar 5, 2013·The Journal of Clinical Investigation·Ryo HottaHeather M Young
Jul 13, 2014·The Journal of Surgical Research·Justin P WagnerJames C Y Dunn
Sep 10, 2014·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D NatarajanA J Burns
Oct 28, 2014·Stem Cells·Kyeongmi KimSergei Y Sokol
Nov 7, 2014·Frontiers in Neuroscience·Yuki Fujita, Toshihide Yamashita
Aug 16, 2015·Pediatric Surgery International·David CoylePrem Puri
Sep 4, 2015·The Journal of Physiological Sciences : JPS·Kei GotoMiyako Takaki
Nov 17, 2015·The Journal of Clinical Investigation·Robert O Heuckeroth
Nov 17, 2015·The Journal of Clinical Investigation·Rodolphe SoretUNKNOWN Ente-Hirsch Study Group
Apr 10, 2016·Developmental Biology·Alan J BurnsPieter Vanden Berghe
Dec 21, 2017·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Qian JiangLong Li

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Feb 9, 2021·Frontiers in Cell and Developmental Biology·Laura E KuilMaria M Alves

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