Inhibition of S-phase entry of human fibroblasts by an antisense oligomer against hCDC47

Biochemical and Biophysical Research Communications
M FujitaM Ishibashi

Abstract

The MCM protein family has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Recently, we have identified human (h) CDC47, another member of the family, and demonstrated its unique S-phase nuclear association pattern implying participation in the regulation of DNA replication. In the present study, we applied an antisense strategy to assess the phenotype of human cells in which hCDC47 is repressed. As a result serum-induced accumulation of hCDC47 protein in human fibroblast cells was reduced by a specific antisense oligodeoxynucleotide and S-phase entry of such cells was significantly inhibited, indicating that hCDC47 protein is essential for DNA replication.

Citations

Jan 16, 2003·Nucleic Acids Research·Devrim GozuacikPatrizia Paterlini-Bréchot
Jul 1, 1997·European Journal of Biochemistry·A Richter, R Knippers
Aug 7, 1998·Journal of Cutaneous Pathology·A HiraiwaM Ishibashi
Jan 1, 1997·Annual Review of Cell and Developmental Biology·A Dutta, S P Bell
Dec 15, 2010·Acta histochemica·Sarah E HensonCindy S Malone
Aug 31, 2000·Annual Review of Biochemistry·T J Kelly, G W Brown
Jan 6, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Mumtaz V RojianiAmyn M Rojiani
Apr 18, 1997·The Journal of Biological Chemistry·M FujitaM Ishibashi
Apr 22, 1997·International Journal of Cancer. Journal International Du Cancer·A HiraiwaM Ishibashi
Aug 5, 1998·Biochimica Et Biophysica Acta·S E Kearsey, K Labib

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.