Inhibition of Salmonella Binding to Porcine Intestinal Cells by a Wood-Derived Prebiotic

Microorganisms
Aleksandar BožićDavid J Nisbet

Abstract

Numerous Salmonellaenterica serovars can cause disease and contamination of animal-produced foods. Oligosaccharide-rich products capable of blocking pathogen adherence to intestinal mucosa are attractive alternatives to antibiotics as these have potential to prevent enteric infections. Presently, a wood-derived prebiotic composed mainly of glucose-galactose-mannose-xylose oligomers was found to inhibit mannose-sensitive binding of select SalmonellaTyphimurium and Escherichia coli strains when reacted with Saccharomyces boulardii. Tests for the ability of the prebiotic to prevent binding of a green fluorescent protein (GFP)-labeled S.Typhimurium to intestinal porcine epithelial cells (IPEC-J2) cultured in vitro revealed that prebiotic-exposed GFP-labeled S.Typhimurium bound > 30% fewer individual IPEC-J2 cells than did GFP-labeled S.Typhimurium having no prebiotic exposure. Quantitatively, 90% fewer prebiotic-exposed GFP-labeled S.Typhimurium cells were bound per individual IPEC-J2 cell compared to non-prebiotic exposed GFP-labeled S.Typhimurium. Comparison of invasiveness of S.Typhimurium DT104 against IPEC-J2 cells revealed greater than a 90% decrease in intracellular recovery of prebiotic-exposed S.Typhimurium DT104 compared ...Continue Reading

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