PMID: 9555096Jun 20, 1998Paper

Inhibition of secreted phospholipases A2 by annexin V. Competition for anionic phospholipid interfaces allows an assessment of the relative interfacial affinities of secreted phospholipases A2

Biochimica Et Biophysica Acta
A G Buckland, D C Wilton

Abstract

The ability of annexins, particularly annexin 1 (lipocortin 1), to inhibit phospholipase A2 (PLA2) is well known and a substrate depletion mechanism is now widely accepted as the explanation for most inhibitory studies. In this investigation we have examined the substrate depletion mechanism of annexin V using a variety of phospholipid substrates and secreted PLA2's (sPLA2). The results suggest that the term interfacial competition best describes the inhibitory effect of annexin V although the overall inhibitory process remains one of substrate sequestration by the annexin. We have utilised the competitive nature of the interaction of enzyme and annexin V for a phospholipid interface as a means of quantifying the relative affinity of sPLA2's for anionic phospholipid vesicles. The results highlight the very high affinity of the human non-pancreatic sPLA2 for such vesicles (Kd<10-(10) M) while the Naja naja venom PLA2 and porcine pancreatic sPLA2 showed lower affinities. Hydrolysis of mixed vesicles containing phosphatidylserine and phosphatidylcholine by the venom and pancreatic enzymes were differentially inhibited by annexin V. This difference must reflect the preference of both annexin V and the pancreatic enzyme for an anion...Continue Reading

References

Aug 20, 1992·Journal of Molecular Biology·C RavanatJ M Freyssinet
Oct 1, 1992·Archives of Biochemistry and Biophysics·J F Tait, D Gibson
Mar 19, 1991·Biochemistry·P MeersD Papahadjopoulos
Jan 1, 1991·Proteins·F Ramirez, M K Jain
Jul 1, 1990·The Journal of Cell Biology·D D Schlaepfer, H T Haigler
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F HirataJ Axelrod
Jan 1, 1995·Annual Review of Biochemistry·M H GelbO G Berg
Aug 1, 1994·Biochemical Society Transactions·A Creaney, D C Wilton
Aug 1, 1994·Biochemical Society Transactions·A R Kinkaid, D C Wilton
Feb 11, 1994·Journal of Molecular Biology·C PigaultA Brisson
Nov 1, 1993·Analytical Biochemistry·F M MegliE Quagliareillo

❮ Previous
Next ❯

Citations

Mar 27, 2003·Toxicon : Official Journal of the International Society on Toxinology·I Birgitta SundellR David G Theakston
Jan 17, 2012·Journal of Proteome Research·Joao A PauloHanno Steen
May 19, 2005·Arthritis Research & Therapy·René J BerckmansPaul P Tak
Jan 30, 2013·International Journal of Molecular Sciences·María Antonia LizarbeJavier Turnay
Jun 29, 2004·Muscle & Nerve·Stefan Probst-CousinDieter Heuss
Aug 11, 2007·Chembiochem : a European Journal of Chemical Biology·Maria Chiara MontiRaffaele Riccio
May 4, 2006·Chembiochem : a European Journal of Chemical Biology·Maria Chiara MontiLuigi Gomez-Paloma
Jun 15, 2012·Molecular Membrane Biology·Joanna Bandorowicz-PikulaSlawomir Pikula
May 9, 2002·American Journal of Physiology. Cell Physiology·Xiuqiong WangJohn R Dedman
Feb 24, 2001·Annals of the New York Academy of Sciences·L Miele
Apr 3, 2019·Nature Chemical Biology·Rebecca J EdgarNatalia Korotkova
Jan 15, 2000·Biochimica Et Biophysica Acta·A G Buckland, D C Wilton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.