Inhibition of skeletal muscle S1-myosin ATPase by peroxynitrite

Biochemistry
Teresa TiagoCarlos Gutiérrez-Merino

Abstract

Exposure of myosin subfragment 1 (S1) to 3-morpholinosydnonimine (SIN-1) produced a time-dependent inhibition of the F-actin-stimulated S1 Mg(2+)-ATPase activity, reaching 50% inhibition with 46.7 +/- 8.3 microM SIN-1 for 8.7 microM S1, that is, at a SIN-1/S1 molar ratio of approximately 5.5. The inhibition was due to the peroxynitrite produced by SIN-1 decomposition because (1) decomposed SIN-1 was found to have no effect on S1 ATPase activity, (2) addition of SIN-1 in the presence of superoxide dismutase and catalase fully prevented inhibition by SIN-1, and (3) micromolar pulses of chemically synthesized peroxynitrite produced inhibition of F-actin-stimulated S1 Mg(2+)-ATPase activity. In parallel, SIN-1 produced the inhibition of the nonphysiological Ca(2+)-dependent and K(+)/EDTA-dependent S1 ATPase activity of S1 and, therefore, suggested that the inhibition of F-actin-stimulated S1 Mg(2+)-ATPase activity is produced by the oxidation of highly reactive cysteines of S1 (Cys(707) and Cys(697)), located close to the catalytic center. This point was further confirmed by the titration of S1 cysteines with 5,5'-dithiobis(2-nitrobenzoic acid) and by the parallel decrease of Cys(707) labeling by 5-(iodoacetamido)fluorescein, and i...Continue Reading

References

Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·E FreireJ M Sanchez-Ruiz
May 28, 1969·Journal of Molecular Biology·S LoweyH Baker
May 15, 1995·FEBS Letters·H Ischiropoulos, A B al-Mehdi
Jul 5, 1996·The Journal of Biological Chemistry·I Rayment
Feb 3, 1997·Biochemical and Biophysical Research Communications·T NagasawaD D Kitts
Feb 15, 1997·The Biochemical Journal·C QuijanoR Radi
Oct 24, 1997·Journal of Applied Physiology·W J PerkinsG C Sieck
Jun 6, 1998·The Journal of Biological Chemistry·T GruneK J Davies
Jun 29, 2000·The Journal of Biological Chemistry·M M VanDuijnJ Van Der Zee
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F H AndradeH Westerblad
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Kirsch, H De Groot
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Isabella Dalle-DonneRoberto Colombo
Jun 23, 2004·European Journal of Biochemistry·Yolanda Gutiérrez-MartínFernando Henao

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Citations

Jul 31, 2013·The International Journal of Biochemistry & Cell Biology·Jessica R TerrillPeter G Arthur
Jan 25, 2011·The Journal of Biological Chemistry·Marius P Sumandea, Susan F Steinberg
Oct 21, 2011·Antioxidants & Redox Signaling·David PimentelMarkus Michael Bachschmid
Jan 19, 2013·Circulation Research·Susan F Steinberg
Oct 31, 2009·Arthritis and Rheumatism·Takashi YamadaHåkan Westerblad
Nov 30, 2007·Free Radical Biology & Medicine·Jeremy H SnookWilliam H Guilford
Apr 26, 2013·Acta Physiologica·D G Allen
Nov 3, 2010·The Journal of Physiology·Graham D Lamb, Håkan Westerblad
Sep 15, 2009·The Biochemical Journal·Leonardo NogueiraMartha M Sorenson
Feb 11, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Ricardo LagoaCarlos Gutierrez-Merino
Nov 7, 2014·Journal of Muscle Research and Cell Motility·Lisa Beckendorf, Wolfgang A Linke
Apr 3, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Vijay S RaoWilliam H Guilford
Jan 12, 2008·Journal of Applied Physiology·Brian J HardinMichael B Reid
Jun 3, 2011·American Journal of Physiology. Cell Physiology·Reinaldo DiPolo, Luis Beaugé
Nov 16, 2007·American Journal of Physiology. Cell Physiology·Ewa ProchniewiczDavid D Thomas
Sep 1, 2015·Biophysics Reviews·Martin Breitkreuz, Nazha Hamdani
Feb 22, 2013·American Journal of Physiology. Cell Physiology·Genevieve BatesAnne-Marie Lauzon
Feb 9, 2021·American Journal of Physiology. Endocrinology and Metabolism·Sarah J BlackwoodAbram Katz
Oct 13, 2009·Journal of Chemical Theory and Computation·Manuel YáñezJosé Elguero
Feb 17, 2006·Biochemical and Biophysical Research Communications·Teresa TiagoCarlos Gutiérrez-Merino
Dec 22, 2017·The Journal of Physical Chemistry. a·José Luis Casals-SainzÁngel Martín Pendás

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