PMID: 9189830Jun 15, 1997Paper

Inhibition of smooth muscle cell growth in vitro by an antisense oligodeoxynucleotide released from poly(DL-lactic-co-glycolic acid) microparticles

Journal of Biomedical Materials Research
R L CleekAntonios G Mikos

Abstract

We fabricated poly(DL-lactic-co-glycolic acid) (PLGA) 50:50 microparticles loaded with an antisense (AS) oligodeoxy-nucleotide (ODN) against the rat tenascin mRNA and determined the effect in vitro of the AS-ODN released on smooth muscle cell (SMC) proliferation and migration. AS-ODN was entrapped using a double-emulsion-solvent-extraction technique with high efficiency. Release of AS-ODN was characterized by a small initial-burst effect followed by a period of controlled AS-ODN release for up to 20 days. SMC proliferation studies exhibited dose-dependent growth inhibition with AS-ODN-loaded microparticles. Microparticles loaded with scrambled (SC) ODN showed less growth inhibition than AS-ODN. Moreover, only the AS-ODN-loaded microparticles inhibited migration. These results demonstrate the feasibility of entrapping an AS-ODN to rat tenascin in PLGA microparticles for controlled delivery to inhibit SMC proliferation and migration.

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Citations

Mar 29, 2000·Journal of Biomedical Materials Research·L LuA G Mikos
Dec 28, 2012·Amino Acids·Igor JakovcevskiPavle R Andjus
Dec 4, 2003·Biomaterials·Sharon M SagnellaKandice Kottke-Marchant
Sep 29, 2000·Pharmacology & Therapeutics·C Garcia-ChaumontJ Bolard
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Mar 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·N J MeilanderR V Bellamkonda
Dec 7, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elizabeth L HedbergAntonios G Mikos
Oct 6, 1997·Journal of Hepatology·G W McCaughan, G A Bishop
Mar 30, 2005·Journal of Biomaterials Science. Polymer Edition·Hai-Quan MaoKam W Leong
Feb 6, 2002·Clinics in Plastic Surgery·Saleh M Shenaq, Eser Yuksel
Jun 5, 2003·Expert Opinion on Therapeutic Targets·Jenny Godfray, Peter Estibeiro
Jan 24, 2003·Expert Opinion on Therapeutic Targets·Terace M Fletcher
Nov 12, 2016·Lab on a Chip·Yan MaQun Fang
Nov 11, 1999·Antisense & Nucleic Acid Drug Development·S D PutneyG Zon
Aug 11, 2007·Journal of Biomedical Materials Research. Part a·Esther W H BoddeJohn A Jansen
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Jul 26, 2005·Journal of Biomedical Materials Research. Part a·P Quinten RuhéAntonios G Mikos
Aug 9, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sarah A GebbPeter Lloyd Jones
May 14, 2009·Expert Review of Molecular Diagnostics·Jian-Ming LüChangyi Chen
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Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·P Quinten RuheAntonios G Mikos

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