Inhibition of sodium current by chloride channel blocker 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) in guinea pig cardiac ventricular cells

Journal of Cardiovascular Pharmacology
J LiuK Nishi

Abstract

The effects of 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), a potent anion transport blocker, on transmembrane action potentials (APs) and the sodium current (I[Na]) of guinea pig ventricular myocytes were examined by using conventional microelectrode and whole-cell patch-clamp recording techniques. In papillary muscle preparations, DIDS (> or =0.1 mM) suppressed the maximal upstroke velocity (.v[max]) of the AP without significant changes in other AP parameters. Extracellular application of DIDS on single cardiomyocytes isolated from the guinea pig ventricle markedly reduced the peak amplitude of the tetrodotoxin (TTX)-sensitive and voltage-activated sodium current. The concentration-dependent block of DIDS could be expressed by the Hill equation with a Hill coefficient of 0.97 and a dissociation constant of 0.15 mM at a holding potential of (VH) -120 mV. DIDS (0.1 mM) shifted the steady-state inactivation curve for I(Na) toward more negative potentials by 6.0 +/- 0.5 mV and the activation curve to more positive potentials by 5.0 +/- 1.0 mV, although the slope factors were unaffected. With repetitive depolarizing pulses from -120 mV, DIDS produced a use-dependent block on the I(Na). Recovery of I(Na) from inactiv...Continue Reading

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Citations

Jul 18, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·E StreeterE Badoer
Jan 22, 2003·European Journal of Pharmacology·Bing-Mei ZhuKeitaro Hashimoto
Mar 4, 2000·Clinical and Experimental Pharmacology & Physiology·M V KopanitsaI V Komissarov
Aug 25, 2005·Experimental Physiology·Shi-Sheng ZhouYan-Feng Ding
Aug 31, 2007·Journal of Neurochemistry·Hang YaoGabriel G Haddad
Dec 17, 2008·Journal of Neurochemistry·Hang YaoGabriel G Haddad
Mar 24, 2004·Journal of Pharmacological Sciences·Zhong-Fang LaiKatsuhide Nishi
Jan 11, 2001·Journal of Cardiovascular Pharmacology and Therapeutics·C I SpencerR Z Kozlowski
Feb 20, 2004·American Journal of Physiology. Cell Physiology·Xiao-Gang LaiTak-Ming Wong
Jun 17, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Zhong-Fang LaiShokei Kim-Mitsuyama
Sep 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·John J BorgRoland Z Kozlowski
Oct 17, 2002·Journal of Applied Physiology·Shi-Sheng ZhouXin-Liang Ma
Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S PelzerD J Pelzer
Nov 6, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jin XueGabriel G Haddad

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