Inhibition of somatotroph growth and growth hormone biosynthesis by activin in vitro

Molecular Endocrinology
N BillestrupW Vale

Abstract

Activin-A, a homodimeric protein composed of two inhibin beta A-subunits, was first isolated from gonadal fluids based upon its ability to stimulate FSH secretion and biosynthesis, but was also observed to suppress GH secretion. The present report describes the effects of activin on the biosynthesis of GH and the proliferation of pituitary somatotrophs. In pituitary cells cultured in the presence of 0.7 nM activin for 3 days, GH secretion was decreased by 50% compared to the control value. Inhibition of GH biosynthesis, measured by quantitative immunoprecipitation of [35S]methionine-labeled cells, could be observed after 24 h of activin treatment, and maximal (70%) inhibition of GH biosynthesis was observed after 3 days. Activin inhibited basal as well as GH-releasing factor (GRF)-, glucocorticoid-, and thyroid hormone-stimulated GH biosynthesis. Inhibin, which is known to reverse the effect of activin on FSH secretion, did not reverse the effect of activin on GH biosynthesis. Treatment of somatotrophs with activin for 3 days completely inhibited the growth-promoting effect of GRF on somatotrophs. However, no effect of activin on GRF-stimulated expression of the c-fos protooncogene was observed. These data demonstrate that acti...Continue Reading

Citations

Jan 13, 1994·Biochemical Pharmacology·G Morel
Feb 28, 1992·Biochimica Et Biophysica Acta·M M Matzuk, A Bradley
May 1, 1996·Brain Research. Molecular Brain Research·R M SeñarísC Diéguez
Jan 1, 1994·Peptides·H Houben, C Denef
Jan 1, 1991·Molecular and Cellular Endocrinology·D J O'HalloranS R Bloom
Feb 1, 1993·Molecular and Cellular Endocrinology·U MichelJ K Findlay
Jul 14, 2001·Molecular and Cellular Endocrinology·P C GrayW Vale
Jun 29, 2002·Molecular and Cellular Endocrinology·Nicoletta Di SimoneAlessandro Caruso
May 4, 2002·Molecular and Cellular Endocrinology·Ruth Braw-Tal
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R S StruthersW W Vale
Feb 1, 1991·Journal of Neuroendocrinology·P M Plotsky
Jan 1, 1991·Acta Paediatrica Scandinavica. Supplement·I C Robinson
Feb 2, 2008·Endocrinology·Pasquapina CiarmelaWylie Vale
Sep 1, 1996·Endocrinology and Metabolism Clinics of North America·L E CohenS Radovick
Jun 26, 1998·Clinical Endocrinology·U MichelR Nau
Aug 22, 2001·Biology of Reproduction·A J DulebaR Z Spaczynski
Nov 30, 1990·Biochemical and Biophysical Research Communications·U MichelJ K Findlay
Oct 15, 1991·Biochemical and Biophysical Research Communications·U MichelJ K Findlay
Feb 12, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G V Childs, G Unabia
Mar 10, 2001·Clinical and Experimental Pharmacology & Physiology·L M BilezikjianW W Vale
Nov 15, 1997·Microscopy Research and Technique·G V Childs
Apr 1, 1997·Endocrine Reviews·D Ray, S Melmed
Aug 24, 2002·Endocrinology·Amir S KhanRuxandra Draghia-Akli
Oct 11, 2003·Molecular Endocrinology·Chester W BrownMartin M Matzuk
Oct 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Daniel C DanilaAnne Klibanski
Jul 1, 1993·The Journal of Pediatric Endocrinology·F DominguezM F Scanlon
Jul 17, 2020·International Ophthalmology·Umut DağMehmet Fuat Alakuş
Aug 1, 1993·The Journal of Urology·D J Lamb
Aug 1, 1992·Trends in Endocrinology and Metabolism : TEM·L M Bilezikjian, W W Vale
Jun 30, 1992·Biochemical and Biophysical Research Communications·K TasakaO Tanizawa
Dec 1, 1991·Baillière's Clinical Endocrinology and Metabolism·J Webster, M F Scanlon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.