Inhibition of spontaneous induction of lambdoid prophages in Escherichia coli cultures: simple procedures with possible biotechnological applications

BMC Biotechnology
Agata CzyzGrzegorz Węgrzyn

Abstract

Infections of bacterial cultures by bacteriophages are serious problems in biotechnological laboratories. Apart from such infections, prophage induction in the host cells may also be dangerous. Escherichia coli is a commonly used host in biotechnological production, and many laboratory strains of this bacterium harbour lambdoid prophages. These prophages may be induced under certain conditions leading to phage lytic development. This is fatal for further cultivations as relatively low, though still significant, numbers of phages may be overlooked. Thus, subsequent cultures of non-lysogenic strains may be infected and destroyed by such phage. Here we report that slow growth of bacteria decreases deleterious effects of spontaneous lambdoid prophage induction. Moreover, replacement of glucose with glycerol in a medium stimulates lysogenic development of the phage after infection of E. coli cells. A plasmid was constructed overexpressing the phage 434 cI gene, coding for the repressor of phage promoters which are necessary for lytic development. Overproduction of the cI repressor abolished spontaneous induction of the lambda(imm434) prophage. Simple procedures that alleviate problems with spontaneous induction of lambdoid prophage ...Continue Reading

References

Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T GrodzickerH Eisen
Aug 4, 1995·Science·H H McAdams, L Shapiro
Apr 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Thomas
Nov 5, 1999·Journal of Molecular Biology·M Gottesman

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Citations

May 3, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Dai Kitamoto
Jan 15, 2010·FEMS Immunology and Medical Microbiology·Joanna M LośGrzegorz Wegrzyn
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·David L Shis, Matthew R Bennett
Sep 5, 2020·International Journal of Molecular Sciences·Gracja Topka-BieleckaAlicja Węgrzyn
Jan 7, 2003·Applied and Environmental Microbiology·Merete LundeIngolf F Nes
May 17, 2005·Letters in Applied Microbiology·M ŁośG Wegrzyn
Nov 4, 2011·Foodborne Pathogens and Disease·Bożena Nejman-FaleńczykGrzegorz Węgrzyn
Dec 2, 2008·Journal of Applied Microbiology·J Jofre
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sunita GuptaVivek Mittal
Mar 16, 2017·The ISME Journal·Cristina Howard-VaronaMatthew B Sullivan
Jan 21, 2021·Journal of Bacteriology·Josefina León-Félix, Claudia Villicaña
May 27, 2021·Cell Reports·Amany M Al-AnanyAlexander P Hynes

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Methods Mentioned

BETA
PCR

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