Abstract
Malignant mesothelioma is an aggressive tumor arising from mesothelial cells of serous membranes, and forms spheroid-like cell aggregates in pleural and peritoneal effusions. We examined the levels of anoikis, apoptosis induced by the detachment of cells from the extracellur matrix, in suspension culture in the human mesothelioma cell line NCI-H2052. NCI-H2052 cells were adherent in conventional monolayer cultures, but were found to form spheroids in suspension cultures using dishes with ultra-low cell binding capacity. NCI-H2052 cells proliferated in both cultures, but the proliferation rate was markedly lower in suspension cultures than in monolayer cultures. In addition, NCI-H2052 cells in suspension cultures showed little apoptosis, suggesting that the suspension culture induces anoikis resistance. Western blot analysis revealed that suspension cultures induced activation of Src family kinases (SFK) after spheroid formation. Dasatinib, an inhibitor of multi-tyrosine kinases including SFK, abolished anoikis resistance in suspension cultures, indicating that SFK activated by spheroid formation are responsible for anoikis resistance. Cisplatin induced apoptosis in NCI-H2052 cells, but the apoptotic rate was significantly lower...Continue Reading
References
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Sep 1, 1993·Molecular Biology of the Cell·J E MeredithM A Schwartz
Aug 15, 1997·The Biochemical Journal·G M Cohen
Nov 14, 1997·The American Journal of Physiology·W Mueller-Klieser
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Oct 18, 2000·Critical Reviews in Oncology/hematology·L de RidderD de Ridder
Jul 16, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Nicholas J VogelzangPaolo Paoletti
May 26, 2004·Biochemical Society Transactions·A J ValentijnA P Gilmore
Jun 2, 2004·Nature Reviews. Cancer·Timothy J Yeatman
Jun 11, 2005·Current Oncology Reports·Ranjit Kumar Goudar
Aug 2, 2005·Lancet·Bruce W S RobinsonRichard A Lake
Jun 9, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C Marcela Díaz-MonteroBradley W McIntyre
Mar 15, 2008·The Journal of Biological Chemistry·Dario BarboneV Courtney Broaddus
May 20, 2008·Lancet·Nicholas J Vogelzang
Jun 27, 2008·Cancer Letters·Craig D SimpsonAaron D Schimmer
Mar 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anne S TsaoHedy Lee Kindler
Apr 4, 2009·Cell Death and Differentiation·J DaubriacM-C Jaurand
Aug 28, 2010·Journal of Cellular Physiology·Ryoji EguchiTakashi Nakano
Oct 26, 2011·Cellular Signalling·Xiaoling Zhong, Frederick J Rescorla
Feb 23, 2012·Carcinogenesis·Ryoji EguchiYoshihiro Fujimori
Mar 20, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Arkadiusz Z DudekUNKNOWN Cancer and Leukemia Group B
May 28, 2013·Cellular Signalling·Ryoji EguchiHiroyasu Ogawa
May 29, 2013·Proteomics·Kazuya AraiTadashi Kondo
Dec 3, 2013·Experimental Cell Research·Ludovic CardunerFranck Carreiras
Jan 11, 2014·PloS One·Jianqun LiaoKunle Odunsi
Nov 22, 2014·Cancer Research·Joshua A McCarrollMaria Kavallaris