Inhibition of stage-specific DNA synthesis in rat spermatogenic cells by polycyclic aromatic hydrocarbons

Chemico-biological Interactions
A GeorgellisJ Rydström

Abstract

Changes in the rate of DNA synthesis in spermatogenic cells after treatment of segments of rat seminiferous tubule at defined stages of epithelial cycle with benzo[a]pyrene (BP) or 7,12-methylbenz[a]anthracene (DMBA) were studied. The incorporation of labeled thymidine into DNA was used as a measure of the rate of DNA synthesis. Very little or no inhibition of DNA synthesis at stages V and VIII of the cycle was observed at BP and DMBA concentrations lower than 100 microM. In contrast, in the presence of added mitochondria and/or microsomes from whole rat testis, 20 microM BP or DMBA inhibited DNA synthesis 5% and 80%, respectively. This inhibition of DNA synthesis was prevented by inhibitors of the cytochrome P-450 system and by free radical scavengers. These results suggest that polycyclic aromatic hydrocarbons (PAH) require metabolic activation in order to inhibit DNA replication in seminiferous tubules. The first step of this biotransformation is cytochrome P-450-dependent and occurs in Leydig cells. However, the metabolites produced in this step may be further metabolized to reactive metabolites by peroxidative pathways in the seminiferous tubules; these latter products may affect DNA replication.

References

Dec 17, 1977·Lancet·D WhortonT H Milby
Jul 10, 1979·Biochemistry·L J Marnett, G A Reed
Apr 6, 1978·Biochimica Et Biophysica Acta·J W DePierre, L Ernster
Nov 21, 1977·Biochemical and Biophysical Research Communications·J Van CantfortJ E Gielen
Jan 1, 1989·Chemico-biological Interactions·A Georgellis, J Rydström
Jan 1, 1987·Toxicology and Applied Pharmacology·A GeorgellisJ Rydström
Dec 1, 1988·Carcinogenesis·G A ReedM J Ryan
Jan 1, 1987·Archives of Toxicology·D V Parke
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G A ReedR P Mason
Jan 1, 1986·International Review of Cytology·M ParvinenJ Toppari
Aug 1, 1986·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R P Amann, W E Berndtson
Jan 1, 1974·Advances in Cancer Research·P Sims, P L Grover
Apr 1, 1984·The Biochemical Journal·B Halliwell, J M Gutteridge
Jan 1, 1980·Drug Metabolism Reviews·J DiGiovanni, M R Juchau
Apr 1, 1981·Environmental Health Perspectives·J A Thomas
Feb 1, 1980·The Journal of Cell Biology·H Mori, A K Christensen
Jan 1, 1980·Endocrine Reviews·J P Preslocsk
Sep 30, 1980·Biochemical and Biophysical Research Communications·L J Marnett, M J Bienkowski
Nov 20, 1952·Annals of the New York Academy of Sciences·C P LEBLOND, Y CLERMONT
Sep 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·E V JENSENC HUGGINS
Jun 1, 1978·Environmental Health Perspectives·I P Lee, R L Dixon
Jul 1, 1963·The Journal of Experimental Medicine·E Ford, C Huggins

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Citations

Oct 14, 2003·Reproductive Toxicology·Frank InyangAnthony E Archibong
Dec 14, 2004·Toxicology and Applied Pharmacology·Grantley D CharlesM Sue Marty
Jul 1, 1990·Toxicology and Applied Pharmacology·R E ChapinL T Burka
Mar 28, 2009·Fertility and Sterility·Dyutiman MukhopadhyayAsok K Bhattacharyya
Apr 30, 2008·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Hueiwang Anna Jeng, Liang Yu
May 26, 2016·Environmental Science and Pollution Research International·Salah A SheweitaM Hassan
Oct 23, 2008·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Ryan L WilliamsRobert I Krieger

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