Inhibition of staphylococcal wound infection and potentiation of antibiotic prophylaxis by a recombinant fragment of the fibronectin-binding protein of Staphylococcus aureus

The Journal of Infectious Diseases
Barbara E MenziesD S Kernodle

Abstract

Adherence of Staphylococcus aureus to host tissues is a critical step for colonization and initiation of infection. The fibronectin-binding proteins (FnBPs) of S. aureus have been implicated in adherence and internalization in nonprofessional phagocytes. A recombinant fragment of the fibronectin-binding domains (rFnBF) that potently inhibits S. aureus entry into host cells was generated. To test the hypothesis that rFnBF may attenuate the establishment of infection, the ability of intermuscularly administered rFnBF to prevent abscess formation was determined in a guinea pig model of wound infection. rFnBF exhibited dose-dependent inhibition of abscess formation and, at a 100-microg dose, raised the median infective dose approximately 170-fold, compared with the control. In addition, rFnBF potentiated the benefit of prophylaxis with cefazolin. Thus, exogenous administration of the fibronectin-binding domain of FnBP reduces the risk of staphylococcal abscess formation and should be investigated further as a novel agent for prevention of wound infection.

Citations

Oct 27, 2004·Archives of Dermatological Research·Sompid KintarakSimon A Whawell
May 13, 2011·European Journal of Cell Biology·Christine HoffmannChristof R Hauck
Aug 11, 2005·Molecular Microbiology·Michael E KonkelSteve G Garvis
Jun 5, 2003·Cellular Microbiology·Trent FowlerMagnus Höök
Apr 23, 2004·Molecular Microbiology·Ulrich Schwarz-LinekJennifer R Potts
Aug 14, 2012·Current Opinion in Cell Biology·Christof R HauckPetra Muenzner
Aug 11, 2010·FEMS Microbiology Reviews·Brian HendersonMark A Williams
Nov 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Marco M DominguesNuno C Santos
Jun 6, 2020·Khirurgiia·V V AlipovD R Mustafaeva
Jun 25, 2003·Current Opinion in Infectious Diseases·Barbara E Menzies
Mar 21, 2006·European Journal of Cell Biology·Christof R HauckTim Schmitter

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