Inhibition of STAT6/Anoctamin-1 Activation Suppresses Proliferation and Invasion of Gastric Cancer Cells

Cancer Biotherapy & Radiopharmaceuticals
Guohao LuHongyu Zheng

Abstract

Gastric carcinoma is the most popular cancer worldwide. Anoctamin-1 is a calcium-activated channel and highly expressed in various tumors. A previous study indicated that suppressed Anoctamin-1 expression decreased cancer cell proliferation or migration. As a signal transduction and transcription activator, STAT6 is a novel agonist for Anoctamin-1 promoter. However, its role in tumor cell proliferation or migration remains unclear. Therefore, this study aimed to suppress STAT6 and Anoctamin-1 protein expression in gastric cancer cells to test the inhibitory effects on gastric cancer cell migration or invasion. MTT colorimetry was used to test cell proliferation. Western blot was used to measure STAT6 and Anoctamin-1 expression before and after small interfering RNA (siRNA) treatment. A scratch assay was performed to measure cell migration, followed by Transwell chamber assay analysis of cell invasion. After STAT6 siRNA interference, the expression of STAT6 and Anoctamin-1 was significantly decreased in the gastric carcinoma cell line. Anoctamin-1 siRNA interference only decreased its protein expression, but not STAT6 protein expression. Interference of STAT6 or Anoctamin-1 reduced their protein expression and inhibited prolifer...Continue Reading

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Citations

Apr 1, 2020·Annals of Surgical Oncology·Keita KatsuraharaEigo Otsuji
Mar 10, 2020·Acta Biochimica Et Biophysica Sinica·Rongjun XieYang Hu
Oct 23, 2019·World Journal of Gastroenterology : WJG·Kyle J AndersonPatricia M Scott
Dec 16, 2021·Omics : a Journal of Integrative Biology·Talip Yasir DemirtasEsra Gov

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Methods Mentioned

BETA
transfection
electrophoresis

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