PMID: 16658684Feb 1, 1974Paper

Inhibition of Sterol Biosynthesis in Chlorella sorokiniana by Triparanol

Plant Physiology
J T Chan, G W Patterson

Abstract

When Chlorella sorokiniana was cultured in the presence of 1 mg/1 triparanol succinate, there was a 42% reduction in total sterol concentration. Algal biomass was reduced by approximately the same amount. In addition to the cycloartenol, cyclolaudenol, 24-methyl-pollinastanol, ergosta-5, 7-dien-3beta-ol, and ergosterol that occur in control culture, pollinastanol, 14alpha-methyl-5alpha-ergost-8-en-3beta-ol, 5alpha-ergosta-8, 14, 22-trien-3beta-ol, 5alpha-ergosta-8(14), 22-dien-3beta-ol, 5alpha-ergosta-8(9), 22-dien-3beta-ol, 5alpha-ergosta-8, 14-dien-3beta-ol, 5alpha-ergost-8(9)-3n-3beta-ol, 5alpha-ergost-8(14)-en-3beta-ol, 5alpha-ergosta-7, 22-dien-3beta-ol, and 5alpha-ergost-7-en-3beta-ol were isolated and identified from triparanol succinate-treated cells. A biosynthetic pathway for sterol biosynthesis in this organism is postulated based on all the sterols that were isolated and identified in triparanol-treated cultures of C. sorokiniana. Cyclolaudenol appears to be the product of the first alkylation at C-24 in this organism rather than the more common 24-methylene cycloartanol. Since 24-methylene sterols are needed for the second alkylation reaction, this would explain the absence of C-29 sterols in C. sorokiniana. Four o...Continue Reading

References

Feb 15, 1972·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·G J SchroepferJ Vermillion
Oct 1, 1970·Steroids·David M Orcutt, B Richardson
Apr 29, 1969·Biochemical and Biophysical Research Communications·B N Lutsky, G J Schroepfer
Nov 1, 1959·Archives of Biochemistry and Biophysics·T R BLOHM, R D MACKENZIE
Jan 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·S AARONSONH BAKER
Sep 1, 1973·Plant Physiology·J T Chan, G W Patterson

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