Inhibition of system A amino acid transport and hepatocyte proliferation following partial hepatectomy in the rat

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
T L FreemanM E Mailliard

Abstract

System A, the sodium-dependent neutral amino acid transport activity, has a 3-fold increase in its initial uptake velocity into hepatocytes following partial hepatectomy (PH) in the rat. The purpose of this study was to examine the effect of inhibition of System A-mediated amino acid transport on hepatocyte proliferation and liver regeneration. We describe the in vivo competitive inhibition of System A activity following PH by the nonmetabolizable, System A-specific substrate, alpha-(methylamino)isobutyric acid (MeAIB). Administration of MeAIB 60 minutes before PH decreased the incorporation of [(3)H]thymidine into DNA by 45% +/- 5% and 76% +/- 17% at 24 and 36 hours, respectively. The readministration of MeAIB every 12 hours further decreased DNA synthesis by 92% +/- 18% and 82% +/- 11% at 24 and 36 hours. The recovery of liver mass of rats receiving MeAIB was decreased by 46.4% +/- 5.1% at 24 hours after PH. In vitro, 5 mmol/L MeAIB inhibited proliferation of primary hepatocytes by 56% +/- 4% and 61% +/- 12% 48 hours after incubation with 10% fetal calf serum or epidermal growth factor (5 ng/mL), respectively. Thus, MeAIB inhibition of System A transport activity decreased both in vivo and in vitro inducement of hepatocyte pr...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F C FowlerM E Mailliard
Nov 15, 1991·The Biochemical Journal·Stephan vom DahlD Häussinger
Dec 12, 1991·Biochimica Et Biophysica Acta·D Häussinger, F Lang
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N X QianE Englesberg
Oct 8, 1970·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·D F ScottV R Potter
Oct 1, 1972·Journal of Cellular Physiology·G Sander, A B Pardee
Jan 23, 1973·Biochemical and Biophysical Research Communications·J ShortI Lieberman
Mar 1, 1968·Biochimica Et Biophysica Acta·H N Christensen, A M Cullen
Mar 1, 1995·Gastroenterology·M E MailliardG E Mann
May 15, 1994·The Biochemical Journal·P G PetroniniK P Wheeler
Sep 1, 1995·Journal of Hepatology·H S BrandR A Chamuleau
Jun 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·O BussolatiG C Gazzola
Nov 22, 1996·The Journal of Biological Chemistry·K S AulakM Hatzoglou
Apr 4, 1997·Science·G K Michalopoulos, M C DeFrances

Citations

Aug 14, 2001·Journal of Cellular Biochemistry·R D KimR S Chari
Oct 4, 2005·Journal of Molecular Histology·Michael D WeissKevin J Anderson
Mar 22, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Young C KimSang K Kim
Jun 11, 2002·Archives of Biochemistry and Biophysics·T L FreemanM E Mailliard
May 7, 2002·Biochemical Pharmacology·T L FreemanM E Mailliard
Jul 12, 2003·Brain Research. Developmental Brain Research·Michael D WeissKevin J Anderson
Jun 18, 2003·The Journal of General Physiology·M López-FontanalsM Pastor-Anglada
Jun 1, 2006·Acta Physiologica·R Franchi-GazzolaO Bussolati
Apr 9, 2008·World Journal of Gastroenterology : WJG·Yoshiyuki TakaharaShuhei Nishiguchi
Nov 30, 2000·Biochemical and Biophysical Research Communications·T L Freeman, M E Mailliard
Feb 10, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sundararajah ThevanantherSaul J Karpen
Dec 14, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Olaf DransfeldMatthias Wettstein
Oct 20, 2007·The FEBS Journal·F SchliessD Häussinger
Jun 15, 2004·Biochemical Pharmacology·T L FreemanM E Mailliard
Oct 22, 2013·The American Journal of Pathology·Jiansheng Huang, David A Rudnick
Oct 17, 2003·British Journal of Cancer·J Ostrowski, K Bomsztyk
Jan 24, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Matteo VidaliEmanuele Albano
Dec 14, 2006·American Journal of Physiology. Endocrinology and Metabolism·Kashif SheikhNicholas D Oakes
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Stuart CramerDonald Novak
Mar 15, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anna Alvarez-GuaitaCarles Rentero
Dec 3, 2020·Journal of Cell Science·Leyao ShenCourtney M Karner

Related Concepts

Me-AIB
Abufne
Citrate de Betaine UPSA
Carrier Proteins
Cell Division Phases
DNA, Double-Stranded
Hepatectomy
Liver
Liver Regeneration
Protein Biosynthesis

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.