Inhibition of target of rapamycin signaling and stress activate autophagy in Chlamydomonas reinhardtii.

Plant Physiology
María Esther Pérez-PérezJosé L Crespo

Abstract

Autophagy is a catabolic membrane-trafficking process whereby cells recycle cytosolic proteins and organelles under stress conditions or during development. This degradative process is mediated by autophagy-related (ATG) proteins that have been described in yeast, animals, and more recently in plants. In this study, we report the molecular characterization of autophagy in the unicellular green alga Chlamydomonas reinhardtii. We demonstrate that the ATG8 protein from Chlamydomonas (CrATG8) is functionally conserved and may be used as a molecular autophagy marker. Like yeast ATG8, CrATG8 is cleaved at the carboxyl-terminal conserved glycine and is associated with membranes in Chlamydomonas. Cell aging or different stresses such as nutrient limitation, oxidative stress, or the accumulation of misfolded proteins in the endoplasmic reticulum caused an increase in CrATG8 abundance as well as the detection of modified forms of this protein, both landmarks of autophagy activation. Furthermore, rapamycin-mediated inhibition of the Target of Rapamycin signaling pathway, a major regulator of autophagy in eukaryotes, results in identical effects on CrATG8 and a relocalization of this protein in Chlamydomonas cells similar to the one observ...Continue Reading

References

Mar 28, 1998·The Journal of Biological Chemistry·T Noda, Y Ohsumi
Oct 6, 1998·Nature·N MizushimaY Ohsumi
Oct 20, 1999·The Journal of Cell Biology·T KirisakoY Ohsumi
Sep 20, 2000·The Journal of Cell Biology·Y KamadaY Ohsumi
Dec 2, 2000·Nature·Y IchimuraY Ohsumi
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Benoît MenandChristophe Robaglia
Jun 19, 2002·The Journal of Biological Chemistry·Jed H DoellingRichard D Vierstra
Dec 21, 2004·Biochemical and Biophysical Research Communications·Dorothée DeprostChristian Meyer
Mar 4, 2005·Molecular Biology of the Cell·Yukiko KabeyaYoshinori Ohsumi
Apr 23, 2005·BMC Biology·Garrett H AndersonMaureen R Hanson
Apr 30, 2005·The Plant Journal : for Cell and Molecular Biology·Yan XiongDiane C Bassham
Apr 30, 2005·The Plant Journal : for Cell and Molecular Biology·Anthony L ContentoDiane C Bassham
Jul 26, 2005·Plant Physiology·Allison R ThompsonRichard D Vierstra
Aug 12, 2006·The Journal of Biological Chemistry·Tomohiro YorimitsuDaniel J Klionsky
Nov 14, 2006·Plant Physiology·Yan XiongDiane C Bassham
Jan 30, 2007·Plant Physiology·Yuki FujikiYoshinori Ohsumi
Aug 19, 2007·Current Opinion in Plant Biology·Diane C Bassham
Oct 3, 2007·Nature Cell Biology·Zhiping Xie, Daniel J Klionsky
Nov 8, 2007·The Journal of Biological Chemistry·Takao HanadaYoshinori Ohsumi
Nov 28, 2007·The Journal of Biological Chemistry·Vanina E AlvarezBoris Turk
Nov 28, 2007·The Journal of Biological Chemistry·Yuko FujiokaFuyuhiko Inagaki
May 30, 2008·Molecular Biology of the Cell·Zhiping XieDaniel J Klionsky
Sep 5, 2008·Molecular Biology of the Cell·Naonobu FujitaTamotsu Yoshimori
Feb 13, 2009·Molecular Biology of the Cell·Nao HosokawaNoboru Mizushima
Feb 14, 2009·Journal of Experimental Botany·Matthias Josef AffenzellerUrsula Lütz-Meindl
Feb 20, 2009·Molecular Biology of the Cell·Yu-Yun Chang, Thomas P Neufeld
Feb 20, 2009·Molecular Biology of the Cell·Chang Hwa JungDo-Hyung Kim
Mar 11, 2009·Biochimica Et Biophysica Acta·Diane C Bassham
Apr 7, 2009·Biochimica Et Biophysica Acta·Eduardo Cebollero, Fulvio Reggiori
May 9, 2009·Nature Reviews. Molecular Cell Biology·Richard D Vierstra

❮ Previous
Next ❯

Citations

Jun 11, 2011·Protoplasma·Tamar Avin-WittenbergGad Galili
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Shiaulou YuanZhaoxia Sun
Jun 30, 2012·Plant Physiology·María Esther Pérez-PérezJosé L Crespo
Aug 6, 2010·PloS One·Yimo Liu, Diane C Bassham
Jan 17, 2012·Annual Review of Plant Biology·Yimo Liu, Diane C Bassham
Dec 18, 2013·Antioxidants & Redox Signaling·Samuel MorisseChristophe H Marchand
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Chia-Hong TsaiChristoph Benning
Aug 1, 2012·Cellular and Molecular Life Sciences : CMLS·Ana Victoria GarciaHeribert Hirt
Mar 7, 2013·Metabolomics : Official Journal of the Metabolomic Society·Takuro ItoMasaru Tomita
Nov 26, 2015·Trends in Plant Science·Simon MichaeliTamar Avin-Wittenberg
Jun 23, 2012·Comptes rendus biologies·Anne Guiboileau, Céline Masclaux-Daubresse
Feb 7, 2012·Current Opinion in Plant Biology·Christophe RobagliaChristian Meyer
Oct 20, 2015·Current Opinion in Plant Biology·Yan Xiong, Jen Sheen
Dec 9, 2014·Plant Signaling & Behavior·Silvia Ramundo, Jean-David Rochaix
Jul 6, 2014·Amino Acids·Cecilia GotorLuis C Romero
Sep 10, 2015·Plant Molecular Biology·Sousuke ImamuraKan Tanaka
Dec 9, 2014·Biochimica Et Biophysica Acta·Jean-David Rochaix, Silvia Ramundo
Dec 9, 2014·Autophagy·María Esther Pérez-PérezStéphane D Lemaire
Apr 23, 2013·Plant Signaling & Behavior·Yunting Pu, Diane C Bassham
Feb 4, 2012·Autophagy·María Esther Pérez-PérezJosé L Crespo
Sep 12, 2012·Autophagy·Daniel J KlionskyBrian Zuckerbraun
Sep 23, 2014·Methods : a Companion to Methods in Enzymology·Diane C Bassham
Apr 26, 2016·The New Phytologist·Adva ShemiAssaf Vardi
Feb 26, 2016·Annual Review of Plant Biology·Thomas DobrenelChristian Meyer
Dec 20, 2012·Annals of Botany·Zhenya P YordanovaElena T Iakimova
Sep 28, 2016·The Plant Journal : for Cell and Molecular Biology·Emily G WerthLeslie M Hicks
Mar 25, 2017·The Journal of Eukaryotic Microbiology·Jürgen VoigtJohannes Wöstemeyer
Feb 24, 2018·Journal of Experimental Botany·Tamar Avin-WittenbergHenri Batoko

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms