Inhibition of TGF-beta1 expression in human peritoneal mesothelial cells by pcDU6 vector-mediated TGF-beta1 shRNA

Nephrology
F LiuXing Chen

Abstract

Transforming growth factor-beta(1) (TGF-beta(1)) is an important mediator of the fibrosis process. High expression of TGF-beta(1) is closely related to peritoneal fibrosis. RNA interference using short hairpin RNA (shRNA) can mediate sequence-specific inhibition of gene expression in mammalian cells. The aim of this study was to assess the effect of shRNA targeting TGF-beta(1) on the expression of TGF-beta(1) in human peritoneal mesothelial cells (HPMC). TGF-beta(1) specific shRNA expression vectors were constructed and introduced to HPMC stimulated with 4.25% D-glucose (Gs) and 10 microg/mL of lipopolysaccharide (LPS). Expression of TGF-beta(1) mRNA was assessed by semiquantification reverse transcription polymerase chain reaction (RT-PCR). The TGF-beta(1) protein level in the culture supernatant was determined by sandwich enzyme-linked immunosorbent assay. The expression of TGF-beta(1) was upregulated significantly in HPMC stimulated with 4.25% D-Gs and 10 microg/mL LPS P < 0.01. TGF-beta(1) expression in pcDU6 plasmid vector-mediated TGF-beta(1) shRNA groups were obviously downregulated when compared to the 4.25% D-Gs and 10 microg/mL LPS group (P < 0.01) and the pcDU6 void vector group (P < 0.05), with no significant differ...Continue Reading

References

Jul 1, 1992·The Journal of Clinical Investigation·W A Border, E Ruoslahti
Jun 1, 1990·Kidney International·E StylianouJ D Williams
Nov 10, 1994·The New England Journal of Medicine·W A Border, N A Noble
Dec 14, 1999·The American Journal of Pathology·M V CronauerF A Offner
Sep 27, 2000·Journal of the American Society of Nephrology : JASN·María M ArrieroAntonio López-Farré
Feb 17, 2001·Journal of Applied Toxicology : JAT·S Levine, A Saltzman
Aug 28, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J F MedcalfK P Harris
Jan 24, 2002·Journal of the American Society of Nephrology : JASN·John D WilliamsGeraint T Williams
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Apr 18, 2002·Genes & Development·Patrick J PaddisonDouglas S Conklin
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Guangchao SuiYang Shi
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jenn-Yah YuDavid L Turner
May 1, 2002·Nature Biotechnology·Cynthia P PaulDavid R Engelke
May 10, 2002·Nucleic Acids Research·Olivier Donzé, Didier Picard

❮ Previous
Next ❯

Citations

Oct 2, 2007·Current Opinion in Oncology·Michael Pennison, Boris Pasche
Feb 27, 2009·Journal of Immunotherapy·Pu LiuKarl Erik Hellström

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Fang YuanLiang-qun Huang
Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis
J WitowskiD G Oreopoulos
Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis
S D BirdR J Walker
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
Jian Qiang Huang, Can-Mao Xie
© 2021 Meta ULC. All rights reserved