Inhibition of the cellular metabolism of Caco-2 cells by prolamin peptides from cereals toxic for coeliacs

Toxicology in Vitro : an International Journal Published in Association with BIBRA
C GiovanniniM De Vincenzi

Abstract

Peptic-tryptic (PT) digests of prolamins derived from several cereals were tested on differentiated Caco-2 cells to study the effect on cellular metabolism, particularly on DNA, RNA, protein and glycoprotein synthesis. Cell viability was evaluated after treatment with the same cereals. Whereas PT digests from bovine serum albumin and both durum wheat types (diploid and tetraploid) did not exert any effect, bread wheat, oats, barley and rye exerted an inhibitory effect close to 80% for DNA and RNA synthesis and close to 60% for (glyco)protein synthesis. Cell viability evaluated by MTT tests did not show any differences between treated and untreated cells. These observations, and previous results, suggest that, whereas prolamin-derived peptides from bread wheat, barley, rye and oats did not cause an immediate cytotoxic effect, they, were however, responsible for cell damage impairing cell metabolism.

References

Mar 13, 1976·Lancet·M M Weiser, A P Douglas
Dec 1, 1985·Journal of Pediatric Gastroenterology and Nutrition·S AuricchioV Silano
Jun 15, 1984·Biochemical and Biophysical Research Communications·S AuricchioV Silano
Jun 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C GiovanniniM De Vincenzi
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O VincentiniF Zucco

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Citations

Nov 15, 2003·Toxicology Letters·Luca ElliMaria Teresa Bardella
Apr 20, 2000·Toxicology·C GiovanniniM De Vincenzi
Nov 15, 2006·Clinical and Experimental Immunology·W SaklyS El Alaoui
Sep 30, 2008·Clinical and Experimental Immunology·D BernardoE Arranz
Dec 4, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D Mitić-CulafićM Filipic
May 19, 2012·Nutrients·Gianna FerrettiLetizia Saturni
Jul 29, 2015·World Journal of Gastroenterology : WJG·Luca ElliMaria Teresa Bardella

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