PMID: 8596727Mar 1, 1996Paper

Inhibition of the hyperpolarization-activated current (if) of rabbit SA node myocytes by niflumic acid

Pflügers Archiv : European journal of physiology
E A Accili, Dario DiFrancesco

Abstract

The effects of the amphiphilic substance niflumic acid (NFA) were examined in myocytes isolated from the sino-atrial node of the rabbit heart. NFA (50 and 500 microM), for 30-60 s, produced a reversible negative chronotropic effect by reducing the rate of diastolic depolarization, suggesting an inhibitory effect on the hyperpolarization-activated pacemaker current (if). NFA (from 0.05 to 500 microM) inhibited if by modifying the current kinetics, without alteration of the conductance. This was shown by evidence indicating that: (1) NFA inhibited if during hyperpolarizing pulses to the mid-point of if activation but not at fully activating voltages; (2) the slope and reversal potential of the fully activated current/voltage (I/V) relation were not altered by NFA, indicating no change in slope conductance or ion selectivity; and (3) hyperpolarizing ramp protocols confirmed the lack of action of 50 microM NFA on the fully activated current and a shift of approximately -8 mV. Although similar to inhibition by acetylcholine (ACh), inhibition by NFA was only partly additive with the action of ACh and was not altered by atropine or pertussis toxin, both of which eliminated the action of ACh. The effect of NFA was present after stimula...Continue Reading

References

Jul 1, 1992·The Journal of Membrane Biology·J L Rae, G Farrugia
Jan 1, 1990·Methods in Enzymology·R Greger
Dec 1, 1987·European Heart Journal·D DiFrancesco
Mar 1, 1996·Pflügers Archiv : European journal of physiology·E A Accili, D DiFrancesco

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Citations

Oct 1, 1996·Molecular Neurobiology·T A Cleland
Jul 25, 2003·Journal of Molecular and Cellular Cardiology·Annalisa BucchiDario DiFrancesco
Nov 2, 1999·The Journal of Physiology·A BarbutiD DiFrancesco
Jan 13, 2009·Journal of Molecular and Cellular Cardiology·Andrea BarbutiDario DiFrancesco
Feb 20, 2004·American Journal of Physiology. Cell Physiology·Xiao-Gang LaiTak-Ming Wong
Sep 8, 2011·Journal of Cellular Physiology·Enrique BalderasAlberto Darszon
Aug 12, 2018·British Journal of Pharmacology·Ying CaoPingzheng Zhou
Sep 2, 1999·Journal of Cardiovascular Pharmacology·M RocchettiA Zaza
Aug 25, 2005·Experimental Physiology·Shi-Sheng ZhouYan-Feng Ding
Apr 30, 2014·Nature Chemical Biology·Marco LolicatoAnna Moroni
Jul 9, 2009·Physiological Reviews·Martin BielXiangang Zong
Dec 14, 2001·The Journal of Biological Chemistry·Catherine ProenzaEric A Accili
Apr 19, 2003·The Journal of Physiology·Claudia AltomareDario DiFrancesco
Mar 1, 1996·Pflügers Archiv : European journal of physiology·E A Accili, D DiFrancesco
Oct 17, 2002·Journal of Applied Physiology·Shi-Sheng ZhouXin-Liang Ma
Jun 28, 2017·Scientific Reports·Jesús Espinal-EnríquezGustavo Martínez-Mekler
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yong Zhang, William G Paterson
Sep 29, 2005·European Journal of Pharmacology·Aurélien ChatelierPatrick Bois

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