PMID: 6987666Feb 1, 1980Paper

Inhibition of the initiation of translation by a factor isolated from Escherichia coli cells

Proceedings of the National Academy of Sciences of the United States of America
V L Clark

Abstract

A low molecular weight factor isolated from Escherichia coli cells was found to inhibit protein synthesis in vitro directed by RNA of bacteriophage R-17. The factor also inhibited poly(U)-directed translation, but higher concentrations were required. When the factor was added after R-17 RNA-directed translation was initiated, the onset of inhibition was delayed. Initiation-independent translation of polysomes was not inhibited. The factor inhibited the binding of N-formylmethionyl-tRNAfMet to 70S ribosomes to form the 70S initiation complex, and it released the N-flrmylmethionyl-tRNAfMet from preformed complexes. The factor did not prevent the formation of N-formylmethionylpuromycin. It was concluded that the factor inhibits specifically the initiation of translation.

References

Feb 15, 1977·Journal of Molecular Biology·H InouyeA Torriani
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J Lucas-Lenard, F Lipmann
Feb 1, 1973·Biochemistry·P C TaiB D Davis
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Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·R E WebsterN D Zinder
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Related Concepts

Cell-Free System
Alkalescens-Dispar Group
N-Formylmethionine
Peptide Chain Elongation, Translational
Peptide Chain Initiation, Translational
Polyribosomes
Poly(A) Tail
RNA, Transfer, Amino Acyl
Protein Biosynthesis

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