PMID: 9000430Feb 1, 1997Paper

Inhibition of the L-type calcium channel by the five muscarinic receptors (m1-m5) expressed in NIH 3T3 cells

Pflügers Archiv : European journal of physiology
K E Pemberton, S V Jones

Abstract

Modulation of L-type calcium channels by the five cloned muscarinic receptors was studied by expression of the receptors in NIH 3T3 cells. Application of acetylcholine (ACh) to cells transfected with m1-m5 resulted in a reduction in the L-type calcium current amplitude. Elevations in intracellular cAMP concentrations induced by 8-bromo-cAMP or forskolin resulted in no discernible change in the L-type calcium current. In addition, treatment with Rp-adenosine 3',5'-cyclic monophosphothioate triethylamine (Rp-cAMPS), a protein kinase A (PKA) inhibitor, had no effect on the L-type currents. Conversely, application of phorbol dibutyrate, an activator of protein kinase C (PKC) or 8-bromo-cGMP, an activator of cGMP-dependent protein kinase (PKG), reduced the calcium currents. Incubation of the cells with KT5823, an inhibitor of PKG, resulted in a reduction of the response to 8-bromo-cGMP. The ACh-induced depression of L-type calcium current amplitude was sensitive to pertussis toxin (PTX) in cells transfected with the m2 or m4 receptor subtype. The m2-muscarinic-receptor-induced inhibition of the L-type calcium current was attenuated by preincubation of the cells with 8-bromo-cAMP and was unaffected by KT5823 or by calphostin C. The m...Continue Reading

Citations

Feb 20, 2003·European Journal of Pharmacology·Juan Guo, Geoffery G Schofield
Mar 3, 1998·Progress in Neurobiology·P S GuthP Valli
Apr 1, 2009·The Journal of General Physiology·Mandy L Roberts-Crowley, Ann R Rittenhouse
Dec 15, 2010·Brain Research·Gang Q Li, Manning J Correia
Dec 24, 2002·Biophysical Journal·Roger A BannisterBrett A Adams
Jul 4, 2002·Journal of Psychopharmacology·G NormanH J Little
Dec 16, 2006·Journal of Neuroscience Research·Julio Santos-TorresJavier Yajeya

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