Inhibition of the mitogen-activated protein kinase pathway triggers B16 melanoma cell differentiation.

The Journal of Biological Chemistry
W EnglaroR Ballotti

Abstract

In B16 melanoma cells, mitogen-activated protein (MAP) kinases are activated during cAMP-induced melanogenesis (Englaro, W., Rezzonico, R., Durand-Clément, M., Lallemand, D., Ortonne, J. P., and Ballotti, R. (1995) J. Biol. Chem. 270, 24315-24320). To establish the role of the MAP kinases in melanogenesis, we studied the effects of a specific MAP kinase kinase (MEK) inhibitor PD 98059 on different melanogenic parameters. We showed that PD 98059 inhibits the activation of MAP kinase extracellular signal-regulated kinase 1 by cAMP, but does not impair the effects of cAMP either on the morphological differentiation, characterized by an increase in dendrite outgrowth, or on the up-regulation of tyrosinase that is the key enzyme in melanogenesis. On the contrary, PD 98059 promotes by itself cell dendricity and increases the tyrosinase amount and activity. Moreover, down-regulation of the MAP kinase pathway by PD 98059, or with dominant negative mutants of p21(ras) and MEK, triggers a stimulation of the tyrosinase promoter activity and enhances the effect of cAMP on this parameter. Conversely, activation of the MAP kinase pathway, using constitutive active mutants of p21(ras) and MEK, leads to an inhibition of basal and cAMP-induced ...Continue Reading

References

Apr 1, 1992·The Journal of Investigative Dermatology·H Takahashi, P G Parsons
Nov 20, 1991·JAMA : the Journal of the American Medical Association·N LevineV J Hruby
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E O'Keefe, P Cuatrecasas
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J B GibbsE M Scolnick
Aug 1, 1983·The Journal of Cell Biology·R HalabanA B Lerner
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Dec 30, 1994·Biochemical and Biophysical Research Communications·H SuzukiS Shibahara
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G PagèsJ Pouysségur
Jan 1, 1996·Photochemistry and Photobiology·B A GilchrestM Yaar
Feb 1, 1997·Current Biology : CB·V R Baichwal, P A Baeuerle
Oct 29, 1997·Molecular and Cellular Biology·W XiongM R Rosner

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Citations

Dec 21, 2013·The Journal of Clinical Investigation·Irit AdiniRobert J D'Amato
Mar 29, 2002·International Journal of Cancer. Journal International Du Cancer·Keiran S M Smalley, Tim G Eisen
Jun 8, 2001·Biochemical and Biophysical Research Communications·Q DingB M Evers
Jul 10, 2007·Archives of Pharmacal Research·Dong-Seok KimKyoung-Chan Park
Nov 12, 2003·Archives of Pharmacal Research·Dong-Seok KimKyoung-Chan Park
Apr 20, 2004·Archives of Pharmacal Research·Dong-Seok KimKyoung-Chan Park
Jan 6, 2009·Archives of Dermatological Research·Saebom KimDeokhoon Park
Jun 30, 2005·Cancer Metastasis Reviews·Silke KuphalAnja-Katrin Bosserhoff
Dec 5, 2012·Journal of Natural Medicines·Ippei HoribeYasuo Nagaoka
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Dong-Seok KimKyoung-Chan Park
May 30, 2002·Cellular Signalling·Dong-Seok KimKyoung-Chan Park
Apr 25, 2009·The Journal of Biological Chemistry·Yann CheliRobert Ballotti
Jan 8, 2010·The Journal of Biological Chemistry·Barbara BelleiMauro Picardo
May 23, 2008·The American Journal of Dermatopathology·Jarosław Jóźwiak, Ryszard Galus
May 27, 2005·Journal of Clinical Pathology·C W WongUNKNOWN Cancer Genome Project
Jun 3, 2010·Cancer Research·Carla MilagreRichard Marais
Aug 16, 2011·Journal of Biomedical Science·Hyo-Soon JeongDong-Seok Kim
Jun 17, 2003·Biological & Pharmaceutical Bulletin·Sook-Jung ImWon-Hong Woo
Jun 10, 2004·Biological & Pharmaceutical Bulletin·Yeun-Ja MunWon-Hong Woo
Dec 6, 2005·Biological & Pharmaceutical Bulletin·Dong-Seok KimKyoung-Chan Park
Jan 5, 2007·Biological & Pharmaceutical Bulletin·Dong-Seok KimKyoung-Chan Park
May 3, 2011·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Emi SatoShuji Kojima
Oct 30, 2009·International Journal of Molecular Sciences·Jody P EbanksRaymond E Boissy
Aug 8, 2009·Bioscience, Biotechnology, and Biochemistry·So-Young ChungChang-Seo Park
Jul 10, 2007·Bioscience, Biotechnology, and Biochemistry·Hoon-Seok YoonSe-Jae Kim
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Han-Mo KooGeorge F Vande Woude
Apr 1, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Lean-Teik NgChun-Ching Lin
May 21, 2011·Bioscience, Biotechnology, and Biochemistry·Yeon-Joo KwakJai-Heon Lee
Jun 24, 2006·Bioscience, Biotechnology, and Biochemistry·Kenji OhguchiYoshinori Nozawa
Jan 11, 2013·Oxidative Medicine and Cellular Longevity·Xiaoyu ChenQiusheng Zheng
May 6, 2015·Expert Opinion on Therapeutic Patents·Thanigaimalai PillaiyarSang-Hun Jung
Aug 12, 2015·International Journal of Molecular Sciences·Heun Joo LeeGa-Young Lee
Sep 10, 2014·International Journal of Molecular Sciences·Rosanna Y Y LamChristopher H K Cheng

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