Inhibition of the replication of hepatitis B virus in vitro by oxymatrine

The Journal of International Medical Research
M LinJia-Kun Zheng

Abstract

This study investigated the inhibitory capacity of oxymatrine on in vitro hepatitis B virus (HBV) replication. HepG2.2.15 cells were treated with oxymatrine 50, 100, 200, 400, 800 or 1000 mg/l, or with human interferon-alpha2b (IFN-alpha2b 1000 U/l) as a positive control. Levels of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and HBV-DNA in cell supernatants were determined by enzyme-linked immunosorbent assay and fluorescent quantitative-polymerase chain reaction, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labelling were used to evaluate the cytotoxicity of oxymatrine. The inhibitory effects of oxymatrine gradually increased as the concentration increased from 200 to 1000 mg/l for HBsAg and HBeAg, and from 200 to 400 mg/l for HBV-DNA. There was no inhibitory effect of oxymatrine at concentrations < 200 mg/l. No significant difference was seen between human IFN-alpha2b (positive control) and oxymatrine >or= 200 mg/l. It is concluded that oxymatrine can inhibit in vitro HBV replication and antigen expression at concentrations >or= 200 mg/l.

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Citations

Jul 24, 2012·Chinese Journal of Integrative Medicine·Yi-cheng JianWu-jun Xiong
Apr 27, 2010·World Journal of Gastroenterology : WJG·Wen-Sheng XuJun-Xue Wang
Aug 25, 2012·World Journal of Gastroenterology : WJG·Ning-Li ChaiBen-Yan Wu
Oct 15, 2013·Fundamental & Clinical Pharmacology·Li-Peng QiuKe-Ping Chen
Nov 21, 2012·Fitoterapia·Li-Peng Qiu, Ke-Ping Chen
Aug 9, 2016·Journal of Ethnopharmacology·Yan-Hui SunRen Zhang
Jun 3, 2010·The Journal of International Medical Research·H J Fang, J K Yang
Jun 16, 2011·The Journal of International Medical Research·Z H LuL H Huang
Dec 14, 2018·Medicine·Xiaotao JiangLiang Zheng

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