Inhibition of the STAT3 Signaling Pathway Contributes to the Anti-Melanoma Activities of Shikonin

Frontiers in Pharmacology
Hui-Hui CaoJun-Shan Liu

Abstract

Malignant melanoma is an extremely aggressive and metastatic cancer, and highly resistant to conventional therapies. Signal transducer and activator of transcription 3 (STAT3) signaling promotes melanoma development and progression, which has been validated as an effective target in melanoma treatment. Natural naphthoquinone shikonin is reported to exert anti-melanoma effects. However, the underlying mechanisms have not been fully elucidated. This study aims to evaluate the anti-melanoma activities of shikonin and explore the involvement of STAT3 signaling in these effects. Zebrafish tumor model was established to evaluate the anti-human melanoma effects of shikonin in vivo. MTT assay and colony formation assay were employed to investigate the anti-proliferative effects of shikonin on human melanoma A375 and A2058 cells. Flow cytometry was used to analyze cell cycle distribution and apoptosis induction. Wound healing assay and Transwell chamber assay were conducted to examine the cell migratory and invasive abilities. Immunofluorescence assay was used to observe F-actin, Tubulin, and STAT3 localization. Western blotting was used to determine the expression levels of proteins associated with apoptosis and key proteins in the STA...Continue Reading

References

Apr 14, 2006·Mayo Clinic Proceedings·Deborah L CumminsArjun Chanmugam
Jan 13, 2009·Zebrafish·Craig J CeolLeonard I Zon
Aug 8, 2009·International Journal of Pharmaceutics·Jie HanLi-Min Zhu
Mar 29, 2011·Molecular Interventions·Paul A Johnston, Jennifer R Grandis
Oct 21, 2011·Dermatology Research and Practice·Ichiro YajimaMasashi Kato
Apr 26, 2012·Journal of Natural Products·Nadine KretschmerRudolf Bauer
Oct 26, 2012·Pigment Cell & Melanoma Research·Jose L Orgaz, Victoria Sanz-Moreno
Mar 26, 2013·Journal of Clinical Pathology·Bizhan BandarchiRoya Navab
Oct 16, 2013·Cytoskeleton·Tara Kafiyeh AkhshiAlisa Piekny
Oct 25, 2013·Planta medica·Isabel AndújarMaría Carmen Recio
Nov 28, 2013·Biochemical Pharmacology·Hui-Hui CaoZhi-Ling Yu
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Apr 20, 2014·Cancers·Richard L Carpenter, Hui-Wen Lo
Sep 25, 2014·Investigative Ophthalmology & Visual Science·Wietske van der EntB Ewa Snaar-Jagalska
Dec 19, 2014·Acta Dermato-venereologica·Emanual MaverakisForum Patel
Feb 18, 2015·International Journal of Cancer. Journal International Du Cancer·Qiaoli ZhaoThomas Efferth
Aug 27, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Melissa A WilsonKatherine L Nathanson
Dec 29, 2015·Critical Reviews in Oncology/hematology·Ziqiang ZhuVladimir Gotlieb
Jun 22, 2016·The Journal of Investigative Dermatology·Annalisa SaltariCarlo Pincelli
Aug 30, 2016·Medicinal Research Reviews·Antoine MilletRachid Benhida
Apr 6, 2017·Pigment Cell & Melanoma Research·Ellen van RooijenLeonard I Zon
Jul 1, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Guangyao ZhouYuanping Zhou
Oct 27, 2017·Biochemical Pharmacology·Han-Yue QiuYong-Hua Yang
Dec 6, 2017·Investigative Ophthalmology & Visual Science·Kelly Cristine de Sousa PontesMartine J Jager
Jun 20, 2018·International Journal of Molecular Sciences·Federica LaudisiCarmine Stolfi
Dec 24, 2018·Nature Reviews. Cancer·Jennifer HuynhMatthias Ernst
Mar 13, 2019·Fitoterapia·Fangfang WangJinshan Tang

❮ Previous
Next ❯

Citations

Oct 3, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yu-Qiong HeWan-Sheng Chen
May 4, 2021·Bioorganic Chemistry·Xueyan SunGuotao Tang
Dec 29, 2021·The American Journal of Chinese Medicine·Zhenzhen MuLong Geng

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
xenograft
fluorescence microscopy
Assay
flow cytometry
Transfection
nuclear translocation

Software Mentioned

ModFit LT
Graphpad
Image J
ModFit
Graphpad Prism

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.