Inhibition of the ubiquitin-proteasome system affects influenza A virus infection at a postfusion step.

Journal of Virology
Ivy WidjajaCornelis A M de Haan

Abstract

We have demonstrated that influenza A virus (IAV) RNA synthesis depends on the ubiquitin-proteasome system. IAV replication was reduced both by proteasome inhibitors and in E36ts20 cells, which contain the thermolabile ubiquitin-activating enzyme E1. While virus entry was not affected in E36ts20 cells, the proteasome inhibitor MG132 retained viral particles in the cytoplasm. Addition-removal experiments of MG132 in combination with bafilomycin A1, a well-established inhibitor of IAV entry and fusion, showed that MG132 affected IAV infection at a postfusion step. This was confirmed by the lack of inhibition of IAV entry by proteasome inhibitors in a virus-like particle fusion assay.

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Citations

Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wen-Chi SuMichael M C Lai
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