Inhibition of titanium particle-induced inflammatory osteolysis through inactivation of cannabinoid receptor 2 by AM630

Journal of Biomedical Materials Research. Part a
D GengXian-bin Wang

Abstract

Wear particle could induce inflammatory osteolysis and is the primary pathological factor for aseptic loosening. Although it is known that cannabinoid receptor 2 (CB2) inhibits osteoclast differentiation, the effect on inflammatory osteolysis induced by wear particles remains unclear. This study examined the effect of CB2 in the regulation of osteoclast differentiation in a murine macrophage cell line (RAW264.7), which has been shown to be stimulated by titanium (Ti) particles and receptor activator of the NF-kappaB ligand (RANKL). Results showed that CB2 expression in RAW cells cultured with Ti particles and RANKL. CB2 inactivation by AM630, a CB2 selective antagonist, effectively inhibited osteoclastogenesis in the differentiation medium system. AM630 treatment (> or =100 nM) significantly reduced the number of tartrate-resistant acid phosphatase-positive cells when compared with the control. Real-time reverse transcription polymerase chain reaction analysis revealed that AM630 (100 nM) inhibited mRNA expression of RANK and cathepsin K in RAW cells stimulated by Ti particles and RANKL. Moreover, enzyme-linked immunosorbent assay showed that AM630 (100 nM) reduced protein expression of interleukin-1beta and tumor necrosis fact...Continue Reading

References

Oct 1, 1995·The Journal of Bone and Joint Surgery. American Volume·T P Schmalzried, P Campbell
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·H YasudaT Suda
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L M ChildsE M Schwarz
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y BiE M Greenfield
Jan 5, 2002·Biomaterials·Paul H WooleySam Nasser
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Weiping RenPaul H Wooley
Feb 8, 2003·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jirí GalloRudolf Ditmar
May 16, 2003·Nature·William J BoyleDavid L Lacey
Jan 16, 2004·Gene Therapy·P H Wooley, E M Schwarz
Aug 7, 2004·Endocrinology·Robert J van't HofStuart H Ralston
Dec 29, 2004·Biomaterials·Fabian von KnochMarius von Knoch
Feb 8, 2005·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·D R Haynes
May 24, 2005·Nature Medicine·Aymen I IdrisStuart H Ralston
Aug 9, 2005·The Journal of Bone and Joint Surgery. American Volume·Lawrence D DorrAndrew Yun
Aug 20, 2005·Life Sciences·Dirk G Demuth, Areles Molleman
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Orr OfekItai Bab
Jan 26, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Weiping RenPaul H Wooley
Sep 19, 2006·Clinical Orthopaedics and Related Research·P Edward PurdueThomas P Sculco
Jan 17, 2007·Clinical Orthopaedics and Related Research·Andrew D PearleBryan J Nestor
Apr 19, 2007·Calcified Tissue International·M S Patel, F Elefteriou
May 22, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yasunobu TamakiMichiaki Takagi
Nov 3, 2007·The Journal of Bone and Joint Surgery. American Volume·Kwang Woo NamHee Joong Kim
Dec 12, 2007·British Journal of Pharmacology·I Bab, A Zimmer
Dec 14, 2007·Immunopharmacology and Immunotoxicology·Charles A LunnLoretta Bober
May 23, 2008·European Journal of Pharmacology·Huei-Yann TsaiChih-Hsin Tang
Sep 10, 2008·Neuroscience Letters·Fabiola Voznika MerriamDale E Bjorling
May 5, 2009·The Journal of Bone and Joint Surgery. American Volume·Craig J Della ValleJorge O Galante

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Citations

Jul 6, 2014·Clinical Orthopaedics and Related Research·R Lane Smith, Edward M Schwarz
Sep 17, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ryan T BeckKhaled J Saleh
Sep 14, 2011·Journal of Biomedical Materials Research. Part a·D C GengY Z Xu
Oct 12, 2013·Journal of Biomedical Materials Research. Part a·Ming ChenWei Xu
Jun 28, 2014·The Journal of Surgical Research·Jingfu CuiDechun Geng
Dec 28, 2016·International Journal of Molecular Sciences·Rosaliana LibroOriana Trubiani
Mar 2, 2017·International Journal of Molecular Sciences·Francesca VeronesiMilena Fini
Mar 28, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Guoyin LiuJianning Zhao
May 10, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mireille AlhouayekGiulio G Muccioli
Jul 28, 2020·International Journal of Molecular Sciences·Dongchen AnJan Tytgat
Feb 9, 2018·Future Medicinal Chemistry·Supojjanee SansookRégis Millet

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