Inhibition of TNF-alpha, induced cytokine and adhesion molecule. Expression in glomerular cells in vitro and in vivo by transcription factor decoy for NFkappaB

Experimental Nephrology
Naruya TomitaM Horiuchi

Abstract

Activation of certain cytokines and adhesion molecules has been postulated being involved in the pathogenesis of experimental and human glomerulonephritis. In this study, we examined whether the transcription factor, nuclear factor kappaB (NFkappaB), mediated the expression of these genes involved with the inflammatory response of mesangial cells by using transcription factor decoy oligodeoxynucleotides (ODN) to block NFkappaB binding to the promoter site of its target genes. We hypothesized that the NFkappaB decoy ODN can inhibit the coordinated activation of cytokines and adhesion molecules induced by TNF-alpha. Increased binding activity of NFkappaB induced by TNF-alpha was effectively blocked by the NFkappaB decoy ODN. TNF-alpha stimulated CAT expression, which was significantly inhibited by transfection of NFkappaB, but not by scrambled decoy ODN. Of importance, NFkappaB, but not scrambled decoy ODN, significantly attenuated the increase in RNA and protein levels of IL-1alpha, IL-1beta, IL-6, ICAM-1 and VCAM-1 induced by TNF-alpha assessed by RT-PCR. Moreover, in vivo transfection of NFkappaB decoy ODN inhibited expressions of these cytokines and adhesion molecules induced by TNF-alpha injection. These results suggest a no...Continue Reading

Citations

Oct 6, 2005·Virchows Archiv : an International Journal of Pathology·Ling ZhengStephen I-Hong Hsu
Sep 24, 2005·Clinical and Experimental Immunology·Y UchidaM Kitamura
Aug 30, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yiman MengMasanori Kitamura
Nov 8, 2005·World Journal of Gastroenterology : WJG·Tao Li, Zhen Yang
Dec 7, 2007·International Journal of Cancer. Journal International Du Cancer·Haruhito AzumaYoji Katsuoka
Aug 16, 2016·Journal of the American Society of Nephrology : JASN·Hans-Joachim Anders
Nov 15, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Ryuichi MorishitaToshio Ogihara
Jun 16, 2004·Journal of Neuroscience Research·Jingxin QiuJ Regino Perez-Polo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.